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Protein storage vacuoles originate from remodelled pre-existing vacuoles in Arabidopsis thaliana
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Feeney, Mistianne, Kittelmann, Maike, Menassa, Rima, Hawes, Chris and Frigerio, Lorenzo (2018) Protein storage vacuoles originate from remodelled pre-existing vacuoles in Arabidopsis thaliana. Plant Physiology, 177 (1). pp. 241-255. doi:10.1104/pp.18.00010 ISSN 0032-0889.
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Official URL: http://dx.doi.org/10.1104/pp.18.00010
Abstract
Protein storage vacuoles (PSV) are the main repository of protein in dicotyledonous seeds, but little is known about the origins of these transient organelles. PSV are hypothesised to either arise de novo or to originate from the pre-existing embryonic vacuole (EV) during seed maturation. Here, we tested these hypotheses by studying PSV formation in Arabidopsis (Arabidopsis thaliana) embryos at different stages of seed maturation and recapitulated this process in Arabidopsis leaves reprogrammed to an embryogenic fate by inducing expression of the LEAFY COTYLEDON2 transcription factor. Confocal and immunoelectron microscopy indicated that both storage proteins and tonoplast proteins typical of PSV were delivered to the pre-existing EV in embryos or to the lytic vacuole in reprogrammed leaf cells. In addition, sectioning through embryos at several developmental stages using serial block face scanning electron microscopy revealed the 3D architecture of forming PSV. Our results indicate that the pre-existing EV is reprogrammed to become a PSV in Arabidopsis.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Plant Physiology | ||||||||
Publisher: | American Society of Plant Biologists | ||||||||
ISSN: | 0032-0889 | ||||||||
Official Date: | May 2018 | ||||||||
Dates: |
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Volume: | 177 | ||||||||
Number: | 1 | ||||||||
Page Range: | pp. 241-255 | ||||||||
DOI: | 10.1104/pp.18.00010 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 21 March 2018 |
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