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Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer’s disease subjects
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Everett, James, Collingwood, Joanna F., Tjendana-Tjhin, Vindy, Brooks, Jake, Lermyte, Frederik, Plascencia-Villa, Germán, Hands-Portman, Ian, Dobson, Jon, Perry, George and Telling, Neil D. (2018) Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer’s disease subjects. Nanoscale, 10 (25). 11782-11796 . doi:10.1039/c7nr06794a ISSN 2040-3364.
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Official URL: https://doi.org/10.1039/c7nr06794a
Abstract
Altered metabolism of biometals in the brain is a key feature of Alzheimer’s disease, and biometal interactions with amyloid-β are linked to amyloid plaque formation. Iron-rich aggregates, including evidence for the mixed-valence iron oxide magnetite, are associated with amyloid plaques. To test the hypothesis that increased chemical reduction of iron, as observed in vitro in the presence of aggregating amyloid-β, may occur at sites of amyloid plaque formation in the human brain, the nanoscale distribution and physicochemical states of biometals, particularly iron, were characterised in isolated amyloid plaque cores from human Alzheimer’s disease cases using synchrotron X-ray spectromicroscopy. In situ X-ray magnetic circular dichroism revealed the presence of magnetite: a finding supported by ptychographic observation of an iron oxide crystal with the morphology of biogenic magnetite. The exceptional sensitivity and specificity of X-ray spectromicroscopy, combining chemical and magnetic probes, allowed enhanced differentiation of the iron oxides phases present. This facilitated the discovery and speciation of ferrous-rich phases and lower oxidation state phases resembling zero-valent iron as well as magnetite. Sequestered calcium was discovered in two distinct mineral forms suggesting a dynamic process of amyloid plaque calcification in vivo. The range of iron oxidation states present and the direct observation of biogenic magnetite provide unparalleled support for the hypothesis that chemical reduction of iron arises in conjunction with the formation of amyloid plaques. These new findings raise challenging questions about the relative impacts of amyloid-β aggregation, plaque formation, and disrupted metal homeostasis on the oxidative burden observed in Alzheimer’s disease.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||
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| Subjects: | R Medicine > RC Internal medicine | ||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Library of Congress Subject Headings (LCSH): | Alzheimer's disease -- Causes and theories of causation, Amyloid, Magnetite, Iron | ||||||||||||||||||||||||||||||
| Journal or Publication Title: | Nanoscale | ||||||||||||||||||||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||||||||||||||||||||
| ISSN: | 2040-3364 | ||||||||||||||||||||||||||||||
| Official Date: | 7 July 2018 | ||||||||||||||||||||||||||||||
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| Volume: | 10 | ||||||||||||||||||||||||||||||
| Number: | 25 | ||||||||||||||||||||||||||||||
| Page Range: | 11782-11796 | ||||||||||||||||||||||||||||||
| DOI: | 10.1039/c7nr06794a | ||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 24 April 2018 | ||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 26 April 2018 | ||||||||||||||||||||||||||||||
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