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A suppressor/avirulence gene combination in Hyaloperonospora arabidopsidis determines race specificity in Arabidopsis thaliana
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Woods-Tör, Alison, Studholme, David J., Cevik, Volkan, Telli, Osman, Holub, E. B. and Tör, Mahmut (2018) A suppressor/avirulence gene combination in Hyaloperonospora arabidopsidis determines race specificity in Arabidopsis thaliana. Frontiers in Plant Science, 9 . 265. doi:10.3389/fpls.2018.00265
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Official URL: http://dx.doi.org/10.3389/fpls.2018.00265
Abstract
The pathosystem of Arabidopsis thaliana and diploid biotrophic oomycete Hyaloperonospora arabidopsidis (Hpa) has been a model for investigating the molecular basis of Flor's gene-for-gene hypothesis. The isolates Hpa-Noks1 and Hpa-Cala2 are virulent on Arabidopsis accession RMX-A02 whilst an F1 generated from a cross between these two isolates was avirulent. The F2 progeny segregated 3,1 (avirulent, virulent), indicating a single major effect AVR locus in this pathogen. SNP-based linkage mapping confirmed a single AVR locus within a 14 kb map interval containing two genes encoding putative effectors. The Hpa-Cala2 allele of one gene, designated H. arabidopsidis cryptic1 (HAC1), encodes a protein with a signal peptide and an RxLR/dEER motif, and triggers a defense response in RMX-A02. The second gene is heterozygous in Hpa-Cala2. One allele, designated Suppressor of HAC1Cala2 (S-HAC1Cala2) encodes a protein with a signal peptide and a dKEE motif with no RxLR motif; the other allele (s-hac1 Cala2) encodes a protein with a signal peptide, a dEEE motif and is divergent in sequence from the S-HAC1Cala2 allele. In selfed progeny from Hpa-Cala2, dominant S-HAC1Cala2 allele carrying progeny correlates with virulence in RMX-A02, whereas homozygous recessive s-hac1Cala2 carrying progeny were avirulent. Genetic investigations suggested other heterozygous suppressor loci might exist in the Hpa-Cala2 genome.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||
| Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana -- Genetic aspects, Oomycetes, Plant diseases | |||||||||
| Journal or Publication Title: | Frontiers in Plant Science | |||||||||
| Publisher: | Frontiers Media S.A. | |||||||||
| ISSN: | 1664-462X | |||||||||
| Official Date: | 1 March 2018 | |||||||||
| Dates: |
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| Date of first compliant deposit: | 6 April 2018 | |||||||||
| Volume: | 9 | |||||||||
| Article Number: | 265 | |||||||||
| DOI: | 10.3389/fpls.2018.00265 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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