UNSPECIFIED (2003) Calcium-dependent translocation of cytosolic phospholipase A(2) in pancreatic beta-cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 300 (4). pp. 889-893. ISSN 0006-291X

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Abstract

Cytosolic phospholipase A(2)(cPLA(2)), an enzyme responsible for the generation of arachidonic acid, is located in the cytosolic compartment in most tissues and it translocates, to membrane compartments when activated. We found that cPLA(2) distribution in pancreatic beta-cells is different from that of most other mammalian cells: it is evenly distributed throughout the beta-cell, in both cytoplasmic and nuclear compartments. Agents that increased intracellular Ca2+ in the MIN6 beta-cell line also stimulated a redistribution of cPLA(2) immunoreactivity such that the majority of the enzyme moved from the nucleus to the cytoplasm. The time course of events was compatible with the elevation in Ca2+ being responsible for translocation of cPLA(2). These observations suggest that cPLA(2) may be compartmentalised in unstimulated beta-cells, perhaps to limit its access to substrate prior to elevations in intracellular Ca2+ (C) 2002 Elsevier Science (USA). All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:	0006-291X
Date:	24 January 2003
Volume:	300
Number:	4
Number of Pages:	5
Page Range:	pp. 889-893
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/10088

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