Calcium-dependent translocation of cytosolic phospholipase A(2) in pancreatic beta-cells
UNSPECIFIED. (2003) Calcium-dependent translocation of cytosolic phospholipase A(2) in pancreatic beta-cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 300 (4). pp. 889-893. ISSN 0006-291XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1016/S0006-291X(02)02969-8
Cytosolic phospholipase A(2)(cPLA(2)), an enzyme responsible for the generation of arachidonic acid, is located in the cytosolic compartment in most tissues and it translocates, to membrane compartments when activated. We found that cPLA(2) distribution in pancreatic beta-cells is different from that of most other mammalian cells: it is evenly distributed throughout the beta-cell, in both cytoplasmic and nuclear compartments. Agents that increased intracellular Ca2+ in the MIN6 beta-cell line also stimulated a redistribution of cPLA(2) immunoreactivity such that the majority of the enzyme moved from the nucleus to the cytoplasm. The time course of events was compatible with the elevation in Ca2+ being responsible for translocation of cPLA(2). These observations suggest that cPLA(2) may be compartmentalised in unstimulated beta-cells, perhaps to limit its access to substrate prior to elevations in intracellular Ca2+ (C) 2002 Elsevier Science (USA). All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS|
|Publisher:||ACADEMIC PRESS INC ELSEVIER SCIENCE|
|Date:||24 January 2003|
|Number of Pages:||5|
|Page Range:||pp. 889-893|
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