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The crystal structure and oligomeric form of Escherichia coli L,D-carboxypeptidase A
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Meyer, Karen, Addy, Christine, Akashi, Satoko, Roper, David I. and Tame, Jeremy R. H. (2018) The crystal structure and oligomeric form of Escherichia coli L,D-carboxypeptidase A. Biochemical and Biophysical Research Communications, 499 (3). pp. 594-599. doi:10.1016/j.bbrc.2018.03.195 ISSN 0006-291X.
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Official URL: http://dx.doi.org/10.1016/j.bbrc.2018.03.195
Abstract
Bacterial peptidoglycan is constructed by cross-linking sugar chains carrying pentapeptide building blocks with two d-alanine residues at the C-terminus. Incorporation into the polymer and subsequent breakdown of peptidoglycan releases a tetrapeptide with a single d-alanine residue. Removal of this residue is necessary for the tripeptide to receive a new D-Ala-D-Ala dipeptide in the synthetic pathway, but proteases are generally unable to work with substrates having residues of unusual chirality close to the scissile bond. Processing of the tetrapeptide is carried out by a dedicated ld-carboxypeptidase, which is of interest as a novel drug target. We describe the high resolution crystal structure of the enzyme from E. coli, and demonstrate the dimeric structure is highly conserved.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Peptidoglycans, Bacterial cell walls, Crystals -- Structure, Ultracentrifugation, Carboxypeptidases | ||||||||||
Journal or Publication Title: | Biochemical and Biophysical Research Communications | ||||||||||
Publisher: | Elsevier | ||||||||||
ISSN: | 0006-291X | ||||||||||
Official Date: | 1 May 2018 | ||||||||||
Dates: |
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Volume: | 499 | ||||||||||
Number: | 3 | ||||||||||
Page Range: | pp. 594-599 | ||||||||||
DOI: | 10.1016/j.bbrc.2018.03.195 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 30 April 2018 | ||||||||||
Date of first compliant Open Access: | 5 April 2019 | ||||||||||
RIOXX Funder/Project Grant: |
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