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Comparative genomics and mutational analysis reveals a novel XoxF-utilising methylotroph in the Roseobacter group isolated from the marine environment
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Howat, Alexandra M., Vollmers, John, Taubert, Martin, Grob, Carolina, Dixon, Joanna L., Todd, Jonathan, Chen, Yin, Kaster, Anne-Kristin and Murrell, J. C. (J. Colin) (2018) Comparative genomics and mutational analysis reveals a novel XoxF-utilising methylotroph in the Roseobacter group isolated from the marine environment. Frontiers in Microbiology, 9 . 766. doi:10.3389/fmicb.2018.00766 ISSN 1664-302X.
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Official URL: https://doi.org/10.3389/fmicb.2018.00766
Abstract
The Roseobacter group comprise a significant group of marine bacteria which are involved in global carbon and sulfur cycles. Some members are methylotrophs, using one-carbon compounds as a carbon and energy source. It has recently been shown that methylotrophs generally require a rare earth element when using the methanol dehydrogenase enzyme XoxF for growth on methanol. Addition of lanthanum to methanol enrichments of coastal seawater facilitated the isolation of a novel methylotroph in the Roseobacter group: Marinibacterium anthonyi strain La 6. Mutation of xoxF revealed the essential nature of this gene during growth on methanol and ethanol. Physiological characterisation demonstrated the metabolic versatility of this strain. Genome sequencing revealed that strain La 6 has the largest genome of all Roseobacter group members sequenced to date, at 7.18 Mbp. Multi-locus sequence (MLSA analysis showed that whilst it displays the highest core gene sequence similarity with subgroup 1 of the Roseobacter group, it shares very little of its pangenome, suggesting unique genetic adaptations. This research revealed that the addition of lanthanides to isolation procedures was key to cultivating novel XoxF-utilising methylotrophs from the marine environment, whilst genome sequencing and MLSA provided insights into their potential genetic adaptations and relationship to the wider community.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QR Microbiology | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Marine bacteria, Carbon cycle (Biogeochemistry), Sulfur cycle | ||||||
| Journal or Publication Title: | Frontiers in Microbiology | ||||||
| Publisher: | Frontiers Research Foundation | ||||||
| ISSN: | 1664-302X | ||||||
| Official Date: | 27 April 2018 | ||||||
| Dates: |
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| Volume: | 9 | ||||||
| Article Number: | 766 | ||||||
| DOI: | 10.3389/fmicb.2018.00766 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 18 April 2018 | ||||||
| Date of first compliant Open Access: | 15 June 2018 | ||||||
| RIOXX Funder/Project Grant: |
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