Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Methodological considerations for the identification of choline and carnitine-degrading bacteria in the gut

Tools
- Tools
+ Tools

Jameson, Eleanor, Quareshy, Mussa and Chen, Yin (2018) Methodological considerations for the identification of choline and carnitine-degrading bacteria in the gut. Methods, 149 . pp. 42-48. doi:10.1016/j.ymeth.2018.03.012

[img]
Preview
PDF
WRAP-methodological-considerations-choline-carnitine-degrading-gut-Chen-2018.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (1545Kb) | Preview
[img] PDF
WRAP-methodological-considerations-identification-choline-carnitine-degrading-bacteria-gut-Jameson-2018.pdf - Accepted Version
Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer.

Download (737Kb)
Official URL: http://dx.doi.org/10.1016/j.ymeth.2018.03.012

Request Changes to record.

Abstract

The bacterial formation of trimethylamine (TMA) has been linked to cardiovascular disease. This review focuses on the methods employed to investigate the identity of the bacteria responsible for the formation of TMA from dietary choline and carnitine in the human gut. Recent studies have revealed the metabolic pathways responsible for bacterial TMA production, primarily the anaerobic glycyl radical-containing, choline-TMA lyase, CutC and the aerobic carnitine monooxygenase, CntA. Identification of these enzymes has enabled bioinformatics approaches to screen both human-associated bacterial isolate genomes and whole gut metagenomes to determine which bacteria are responsible for TMA formation in the human gut. We centre on several key methodological aspects for identifying the TMA-producing bacteria and report how these pathways can be identified in human gut microbiota through bioinformatics analysis of available bacterial genomes and gut metagenomes.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
R Medicine > RC Internal medicine
Divisions: Faculty of Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Choline, Carnitine, Gastrointestinal system -- Microbiology, Bacteria, Cardiovascular system -- Diseases
Journal or Publication Title: Methods
Publisher: Academic Press
ISSN: 1095-9130
Official Date: 1 October 2018
Dates:
DateEvent
1 October 2018Published
19 April 2018Available
26 March 2018Accepted
Volume: 149
Page Range: pp. 42-48
DOI: 10.1016/j.ymeth.2018.03.012
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
RPG-2016-307Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
UNSPECIFIED[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us