Baker, Phillip Nigel (1980) A study on the control of interferon production in lymphoblastoid cells. PhD thesis, University of Warwick.

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Abstract

This research was aimed at increasing the yield of lymphoblastoid interferon produced by the Namalwa line of lymphoblastoid cells. The parameters involved in the induction and production of interferon were studied first to give basic biochemical information about interferon and viral production in Namalwa cells. Optimised conditions were used for the remaining studies. The Namalwa cell line was cloned from single cells to determine whether there was any variation in interferon production in a population of cells. No significant variation was found so a study was made with cells treated with 5’-bromodeoxyuridine and butyric acid. These chemicals alter gene activity in many other cell types. The study was made using the mechanism of superinduction as a basic model for the control factors involved in interferon production. Neither chemical showed identical biochemical effects to the anti-metabolites used to effect superinduction in fibroblast cells. Thus it appears that the two chemicals act in a different manner to anti-metabolites. Neither compound affected the replication of the inducing virus, further investigation showed that 5'-bromodeoxyuridine required incorporation into cellular DNA before increased interferon yields were obtained. The effect of butyric acid on interferon yields could not be directly associated with any of its varied biochemical and morphological effects.

These results have increased our knowledge of lymphoblastoid cells and indicated that interferon production in these cells may be controlled in a similar, though not identical way, to the model proposed for fibroblast interferon from fibroblasts. Two chemicals have been shown to increase the yield of interferon from Namalwa cells but the biochemical basis of their activity has not been resolved.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QR Microbiology
Library of Congress Subject Headings (LCSH):	Interferon, Lymphoblastoid cell lines
Official Date:	February 1980
Dates:	Date Event February 1980 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Burke, Derek ; Morser, Michael John
Sponsors:	Science Research Council (Great Britain) ; Wellcome Trust (London, England)
Format of File:	pdf
Extent:	xi, 159 leaves : illustrations
Language:	eng

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