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Smith, Edward M., Green, Laura E. and Mason, David (2003) Savinase is a bactericidal enzyme. Applied and Environmental Microbiology, Vol.69 (No.1). pp. 719-721. doi:10.1128/AEM.69.1.719-721.2003
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Official URL: http://dx.doi.org/10.1128/AEM.69.1.719-721.2003
Abstract
Mastitis is probably the most important disease affecting dairy cows worldwide and has been estimated to cost $1.5 to 2.0 billion/year in the United States alone (4). Although detection methodology continues to improve, microbiological culture is still the recommended method (2) for determining the bacterial content of a milk sample, despite this procedure being both time-consuming and expensive. Consequently, more rapid methods of identification and/or enumeration of bacteria in milk are being developed (3, 5). Many of these techniques, e.g., PCR-based methods, can be completed in a matter of hours, but they lack the specificity to be able to determine the bacterial species present or are unable to enumerate the bacteria present. There can also be a problem with techniques that require a period of enrichment prior to analysis.
Item Type: | Journal Item | ||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology S Agriculture > SF Animal culture T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Mastitis, Cattle -- Diseases -- Research, Staphylococcus aureus infections, Bactericides, Enzymes -- Analysis, Milk -- Microbiology | ||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||
Publisher: | American Society of Microbiology | ||||
ISSN: | 0099-2240 | ||||
Official Date: | January 2003 | ||||
Dates: |
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Volume: | Vol.69 | ||||
Number: | No.1 | ||||
Number of Pages: | 2 | ||||
Page Range: | pp. 719-721 | ||||
DOI: | 10.1128/AEM.69.1.719-721.2003 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Description: | Letter to the editor. Item also includes a response to this letter by Gunasekera, T.S. and Veal, D.A. |
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Version or Related Resource: | Letter in response to: Gunasekera, T.S., Attfield, P.V. and Veal, D.A. (2000). A flow cytometry method for rapid detection and enumeration of total bacteria in milk. Applied and Environmental Microbiology, 66, pp. 1228-1232. |
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