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Profiling the serum protein corona of fibrillar human islet amyloid polypeptide

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Pilkington, Emily H., Gustafsson, Ove Johan Ragnar, Xing, Yanting, Hernandez Fernaud, Juan Ramon, Zampronio, Cleidiane, Kakinen, Aleksandr, Faridi, Ava, Ding, Feng, Wilson, Paul, Ke, Pu Chun and Davis, Thomas P. (2018) Profiling the serum protein corona of fibrillar human islet amyloid polypeptide. ACS Nano, 12 (6). pp. 6066-6078. doi:10.1021/acsnano.8b02346 ISSN 1936-0851.

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Official URL: http://dx.doi.org/10.1021/acsnano.8b02346

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Abstract

Amyloids may be regarded as native nanomaterials that form in the presence of complex protein mixtures. By drawing an analogy with the physicochemical properties of nanoparticles in biological fluids, we hypothesized that amyloids should form a protein corona in vivo that would imbue the underlying amyloid with a modified biological identity. To explore this hypothesis we characterized the protein corona of human islet amyloid polypeptide (IAPP) fibrils in FBS using two complementary methodologies developed herein; quartz crystal microbalance and ‘centrifugal capture’, coupled with nano-liquid chromatography tandem mass spectroscopy. Clear evidence for a significant protein corona was obtained. No trends were identified for amyloid corona proteins based on their physicochemical properties, while strong binding with IAPP fibrils occurred for linear proteins or multi-domain proteins with structural plasticity. Proteomic analysis identified amyloid-enriched proteins that are known to play significant roles in mediating cellular machinery and processing, potentially leading to pathological outcomes and therapeutic targets.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Library of Congress Subject Headings (LCSH): Amyloid, Polypeptides, Nanoparticles
Journal or Publication Title: ACS Nano
Publisher: American Chemical Society
ISSN: 1936-0851
Official Date: 26 June 2018
Dates:
DateEvent
26 June 2018Published
10 May 2018Available
10 May 2018Accepted
Volume: 12
Number: 6
Page Range: pp. 6066-6078
DOI: 10.1021/acsnano.8b02346
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 11 May 2018
Date of first compliant Open Access: 10 May 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
CE140100036Australian Research Councilhttp://dx.doi.org/10.13039/501100000923
1553945National Science Foundationhttp://dx.doi.org/10.13039/100000001
R35GM119691National Institute for Health Researchhttp://dx.doi.org/10.13039/501100000272
Australian Laureate FellowshipAustralian Research Councilhttp://dx.doi.org/10.13039/501100000923
RTP scholarshipAustralia. GovernmentUNSPECIFIED

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