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Manganese is essential for PlcP metallophosphoesterase activity involved in lipid 2 remodelling in abundant marine heterotrophic bacteria
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Wei, Tao, Quareshy, Mussa, Zhang, Yu-zhong, Scanlan, David J. and Chen, Yin (2018) Manganese is essential for PlcP metallophosphoesterase activity involved in lipid 2 remodelling in abundant marine heterotrophic bacteria. Applied and Environmental Microbiology, 84 . e01109-18-e01118. doi:10.1128/AEM.01109-18 ISSN 0099-2240.
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Official URL: https://doi.org/10.1128/AEM.01109-18
Abstract
In vast areas of the ocean, microbes must adapt to the availability of scarce nutrients, and a key strategy for reducing the cellular phosphorus (P) quota is to remodel membranes by replacing phospholipids with non-P surrogate lipids. A metallophosphoesterase, PlcP, is essential for lipid remodeling in cosmopolitan marine bacteria of the Roseobacter (e.g., Phaeobacter sp. strain MED193) and SAR11 (e.g., Pelagibacter sp. strain HTCC7211) clades, and transcription of plcP is known to be induced by P limitation. In order to better understand PlcP-mediated lipid remodeling, we sought to characterize PlcP for its metal ion requirement and to determine its selectivity for native bacterial phospholipids. Here, we report the occurrence of a highly conserved binuclear ion center in PlcPs from MED193 and HTCC7211 and show that manganese is the preferred metal for metallophosphoesterase activity. PlcP displayed high activity towards the major bacterial phospholipids, e.g., phosphatidylglycerol but also phosphatidic acid, a key intermediate in phospholipid biosynthesis. In contrast, phosphatidylserine and phosphatidylinositol, both of which are rare lipids in bacteria, are not preferred substrates. These data suggest that PlcP undertakes a generic lipid remodeling role during the cellular response of marine bacteria to P deficiency and that manganese availability may play a key role in regulating the lipid remodeling process.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||||
Publisher: | American Society for Microbiology | ||||||
ISSN: | 0099-2240 | ||||||
Official Date: | 17 July 2018 | ||||||
Dates: |
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Volume: | 84 | ||||||
Page Range: | e01109-18-e01118 | ||||||
DOI: | 10.1128/AEM.01109-18 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 17 May 2018 | ||||||
Date of first compliant Open Access: | 17 January 2019 | ||||||
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