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Assays to monitor mitophagy in Drosophila
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Tsapras, P., Jacomin, Anne-Claire and Nezis, Ioannis P. (2019) Assays to monitor mitophagy in Drosophila. In: Autophagy. Methods in Molecular Biology, 1880 . New York, NY: Humana Press, pp. 643-653. ISBN 9781493988723
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Official URL: https://doi.org/10.1007/978-1-4939-8873-0_42
Abstract
Autophagy is a central pathway utilized by many eukaryotic cells in order to recycle intracellular constituents, particularly under periods of nutrient scarcity or cellular damage. The process is evolutionary conserved from yeast to mammals and can be highly selective with regards to the contents that are targeted for degradation. The availability of Drosophila transgenic lines and fluorophore-labelled autophagic markers allows nowadays for the more effortless visualization of the process within cells. Herein, we provide two protocols to prepare Drosophila samples for confocal and transmission electron microscopy for in vivo monitoring of mitophagy; a specific type of autophagy for the clearance of damaged or superfluous mitochondria from cells.
Item Type: | Book Item | |||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Series Name: | Methods in Molecular Biology | |||||||||
Journal or Publication Title: | Methods in Molecular Biology | |||||||||
Publisher: | Humana Press | |||||||||
Place of Publication: | New York, NY | |||||||||
ISBN: | 9781493988723 | |||||||||
ISSN: | 1064-3745 | |||||||||
Book Title: | Autophagy | |||||||||
Official Date: | 5 January 2019 | |||||||||
Dates: |
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Volume: | 1880 | |||||||||
Page Range: | pp. 643-653 | |||||||||
DOI: | 10.1007/978-1-4939-8873-0_42 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Date of first compliant deposit: | 22 May 2018 | |||||||||
RIOXX Funder/Project Grant: |
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