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An assessment of candidate genes to assist prognosis in gastric cancer
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McFarlane, Michael J., Brettschneider, Julia, Gelsthorpe, Adrian, James, Sean, Snead, David, Gopalakrishnan, Kishore, Mehenna, Hisham, Jankowski, Janusz A. Z., Arasaradnam, Ramesh P. and Nwokolo, Chuka U. (2018) An assessment of candidate genes to assist prognosis in gastric cancer. Journal of gastrointestinal oncology, 9 (2). pp. 303-310. doi:10.21037/jgo.2018.01.05
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Official URL: https://doi.org/10.21037/jgo.2018.01.05
Abstract
Gastric cancer (GC) is the fourth commonest cancer worldwide, with the second highest mortality rate. Its poor mortality is linked to delayed presentation. There is a drive towards non-invasive biomarker screening and monitoring of many different types of cancer, although with limited success so far. We aimed to determine if any genes from a 32-gene panel could be used to determine GC prognosis. We carried out a retrospective study on the expression of 32 genes, selected for their proven or potential links to GC, on historic formalin fixed paraffin-embedded (FFPE) GC specimens from our unit. Gene expression was measured using quantitative nuclease protection assays (qNPA) technology. Following statistical analysis of the results, immunohistochemical staining for eight genes, both discriminating and non-discriminating, was conducted in seven age and sex matched non-metastatic: metastatic GC pairings. The stained samples were reviewed by two blinded consultant histopathologists. Multivariate Cox analysis of the gene expression data revealed metastatic status, age, sex and five genes appeared to influence GC survival. Genes negatively influencing survival included and (relative risks 2.20, 3.73 and 7.53 respectively). Genes conveying survival benefit included and (relative risks 0.10 and 0.24 respectively). Immunohistochemical staining of seven age and sex matched non-metastatic: metastatic pairs revealed no association between gene expression and protein expression. Our study found several genes whose expression may affect GC prognosis. However, immunohistochemical analysis revealed no association between gene expression and protein expression. It remains to be determined whether gene expression or protein expression are reliable means of assessing GC prognosis.
| Item Type: | Journal Article | ||||||
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| Divisions: | Faculty of Science > Statistics Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | ||||||
| Journal or Publication Title: | Journal of gastrointestinal oncology | ||||||
| Publisher: | AME Publishing Company | ||||||
| ISSN: | 2078-6891 | ||||||
| Official Date: | April 2018 | ||||||
| Dates: |
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| Volume: | 9 | ||||||
| Number: | 2 | ||||||
| Page Range: | pp. 303-310 | ||||||
| DOI: | 10.21037/jgo.2018.01.05 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access |
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