The Library
Kinesin expands and stabilizes the GDP-microtubule lattice
Tools
Tools
Tools
Peet, Daniel R., Burroughs, Nigel John and Cross, R. A. (2018) Kinesin expands and stabilizes the GDP-microtubule lattice. Nature Nanotechnology, 13 (5). pp. 386-391. doi:10.1038/s41565-018-0084-4 ISSN 1748-3395.
|
PDF
WRAP-Kinesin-expands-and-stabilizes-Peet-2018.pdf - Accepted Version - Requires a PDF viewer. Download (10Mb) | Preview |
Official URL: http://dx.doi.org/10.1038/s41565-018-0084-4
Abstract
Kinesin-1 is a nanoscale molecular motor that walks towards the fast-growing (plus) ends of microtubules, hauling molecular cargo to specific reaction sites in cells. Kinesin-driven transport is central to the self-organization of eukaryotic cells and shows great promise as a tool for nano-engineering1. Recent work hints that kinesin may also play a role in modulating the stability of its microtubule track, both in vitro2,3 and in vivo4, but the results are conflicting5,6,7 and the mechanisms are unclear. Here, we report a new dimension to the kinesin–microtubule interaction, whereby strong-binding state (adenosine triphosphate (ATP)-bound and apo) kinesin-1 motor domains inhibit the shrinkage of guanosine diphosphate (GDP) microtubules by up to two orders of magnitude and expand their lattice spacing by ~1.6%. Our data reveal an unexpected mechanism by which the mechanochemical cycles of kinesin and tubulin interlock, and so allow motile kinesins to influence the structure, stability and mechanics of their microtubule track.
| Item Type: | Journal Article | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||||||
| Library of Congress Subject Headings (LCSH): | Kinesin, Microtubules, Eukaryotic cells, Tubulins | ||||||||||||
| Journal or Publication Title: | Nature Nanotechnology | ||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||
| ISSN: | 1748-3395 | ||||||||||||
| Official Date: | 11 March 2018 | ||||||||||||
| Dates: |
|
||||||||||||
| Volume: | 13 | ||||||||||||
| Number: | 5 | ||||||||||||
| Page Range: | pp. 386-391 | ||||||||||||
| DOI: | 10.1038/s41565-018-0084-4 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| Date of first compliant deposit: | 29 May 2018 | ||||||||||||
| Date of first compliant Open Access: | 11 September 2018 | ||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
