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Kinesin expands and stabilizes the GDP-microtubule lattice

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Peet, Daniel R., Burroughs, Nigel John and Cross, R. A. (2018) Kinesin expands and stabilizes the GDP-microtubule lattice. Nature Nanotechnology, 13 (5). pp. 386-391. doi:10.1038/s41565-018-0084-4 ISSN 1748-3395.

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Official URL: http://dx.doi.org/10.1038/s41565-018-0084-4

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Abstract

Kinesin-1 is a nanoscale molecular motor that walks towards the fast-growing (plus) ends of microtubules, hauling molecular cargo to specific reaction sites in cells. Kinesin-driven transport is central to the self-organization of eukaryotic cells and shows great promise as a tool for nano-engineering1. Recent work hints that kinesin may also play a role in modulating the stability of its microtubule track, both in vitro2,3 and in vivo4, but the results are conflicting5,6,7 and the mechanisms are unclear. Here, we report a new dimension to the kinesin–microtubule interaction, whereby strong-binding state (adenosine triphosphate (ATP)-bound and apo) kinesin-1 motor domains inhibit the shrinkage of guanosine diphosphate (GDP) microtubules by up to two orders of magnitude and expand their lattice spacing by ~1.6%. Our data reveal an unexpected mechanism by which the mechanochemical cycles of kinesin and tubulin interlock, and so allow motile kinesins to influence the structure, stability and mechanics of their microtubule track.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Kinesin, Microtubules, Eukaryotic cells, Tubulins
Journal or Publication Title: Nature Nanotechnology
Publisher: Nature Publishing Group
ISSN: 1748-3395
Official Date: 11 March 2018
Dates:
DateEvent
11 March 2018Published
30 January 2018Accepted
Volume: 13
Number: 5
Page Range: pp. 386-391
DOI: 10.1038/s41565-018-0084-4
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 29 May 2018
Date of first compliant Open Access: 11 September 2018
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB-G530233-1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDUniversity of WarwickUNSPECIFIED
103895/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440

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