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Optimized sampling conditions for fecal volatile organic compounds analysis by means of field asymmetric ion mobility spectrometry
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Bosch, Sofie, el Manouni el Hassani, Sofia, Covington, James A., Wicaksono, Alfian, Bomers, Marije K., Benninga, Marc A., Mulder, Chris J. J., de Boer, Nanne K. H and de Meij, Tim G. J. (2018) Optimized sampling conditions for fecal volatile organic compounds analysis by means of field asymmetric ion mobility spectrometry. Analytical Chemistry . doi:10.1021/acs.analchem.8b00688 (In Press)
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WRAP-optimized-fecal-volatile-asymmetric-ion-spectrometry-Covington-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1330Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.analchem.8b00688
Abstract
Background Fecal volatile organic compounds (VOCs) are increasingly considered as potential non-invasive, diagnostic biomarkers for various gastrointestinal diseases. Knowledge of influence of sampling conditions on VOC outcomes is limited. We aimed to evaluate effects of sampling conditions on fecal VOC profiles and to assess under which conditions an optimal diagnostic accuracy in the discrimination between pediatric inflammatory bowel disease (IBD) and controls could be obtained. Methods Fecal samples from de novo treatment-naïve pediatric IBD patients and healthy controls (HC) were used to assess effects of sampling conditions compared to the standard operating procedure (reference standard), defined as 500mg of sample mass, diluted with 10mL tap water, using field asymmetric ion mobility spectrometry (FAIMS). Results A total of 17 IBD (15CD and 2 UC) and 25 HC were included. IBD and HC could be discriminated with high accuracy (accuracy=0.93, AUC=0.99, p<0.0001). Smaller fecal sample mass resulted in a decreased diagnostic accuracy (300mg accuracy=0.77; AUC=0.69, p=0.02; 100mg accuracy=0.70, AUC=0.74, p=0.003). A loss of diagnostic accuracy was seen towards increased numbers of thaw-freeze cycles (one cycle: accuracy=0.61, AUC=0.80, p=0.0004, two cycles: accuracy=0.64, AUC=0.56, p=0.753, three cycles: accuracy=0.57, AUC=0.50, p=0.5101) and when samples were kept at room temperature for 180 minutes prior to analysis (accuracy=0.60, AUC=0.51, p=0.46). Diagnostic accuracy of VOC profiles was not significantly influenced by storage duration differences of 20 months. Conclusion Application of 500mg sample mass analyzed after one thaw-freeze cycle, showed best discriminative accuracy for differentiation of IBD and HC. VOC profiles and diagnostic accuracy were significantly affected by sampling conditions, underlining the need for implementation of standardized protocols in fecal VOC analysis.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry | |||||||||||||||
| Divisions: | Faculty of Science > Engineering | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Chemistry, Organic, Organic compounds, Field ionization mass spectrometry | |||||||||||||||
| Journal or Publication Title: | Analytical Chemistry | |||||||||||||||
| Publisher: | American Chemical Society | |||||||||||||||
| ISSN: | 1520-6882 | |||||||||||||||
| Official Date: | 3 June 2018 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 6 June 2018 | |||||||||||||||
| DOI: | 10.1021/acs.analchem.8b00688 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | In Press | |||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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