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Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein
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Martin, Esther M., Kondrat, Frances D. L., Stewart, Alan J., Scrivens, James H., Sadler, P. J. and Blindauer, Claudia A. (2018) Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein. Scientific Reports, 8 (1). 8646. doi:10.1038/s41598-018-26924-1 ISSN 2045-2322.
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WRAP-native-electrospray-mass-spectrometry-zinc-slycoprotein-Martin-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2855Kb) | Preview |
Official URL: http://doi.org/10.1038/s41598-018-26924-1
Abstract
Zinc modulates the biological function of histidine-rich glycoprotein (HRG) through binding to its His-rich region (HRR). The Zn -binding properties of a 35 amino-acid biologically-active peptide mimic of the HRR, HRGP330, were investigated using dissociative mass spectrometry approaches in addition to travelling-wave ion mobility mass spectrometry (TWIM-MS). Native mass spectrometry confirmed zinc binding to HRGP330; however, broadening of the H NMR resonances upon addition of Zn ions precluded the attainment of structural information. A complementary approach employing TWIM-MS indicated that HRGP330 has a more compact structure in the presence of Zn ions. Top-down MS/MS data supported a metal-binding-induced conformational change, as fewer fragments were observed for Zn -bound HRGP330. Zn -bound fragments of both N-terminal and C-terminal ends of the peptide were identified from collision-induced dissociation (CID) and electron transfer dissociation/proton transfer reaction (ETD/PTR) experiments, suggesting that multiple binding sites exist within this region of HRG. The combination of mass spectrometry and NMR approaches provides new insight into the highly dynamic interaction between zinc and this His-rich peptide.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QP Physiology | ||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Glycoproteins, Zinc, Peptides, Electrospray ionization mass spectrometry | ||||||||||||||||||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||||||||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||||||||||||||
| ISSN: | 2045-2322 | ||||||||||||||||||||||||
| Official Date: | 5 June 2018 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 8 | ||||||||||||||||||||||||
| Number: | 1 | ||||||||||||||||||||||||
| Article Number: | 8646 | ||||||||||||||||||||||||
| DOI: | 10.1038/s41598-018-26924-1 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router. ** History: received 01-03-2018; accepted 17-05-2018. | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
| Date of first compliant deposit: | 18 June 2018 | ||||||||||||||||||||||||
| Date of first compliant Open Access: | 19 June 2018 | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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