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Preparation and characterisation of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size

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Baker, Ysobel R., Chen, Jinfeng, Brown, Jason, El-Sagheer, Afaf H., Wiseman, Philip, Johnson, Errin, Goddard, Paul and Brown, Tom (2018) Preparation and characterisation of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size. Nucleic Acids Research, 46 (15). pp. 7495-7505. doi:10.1093/nar/gky630

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Official URL: https://doi.org/10.1093/nar/gky630

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Abstract

Recently reported DNA nanoflowers are an interesting class of organic-inorganic hybrid materials which are prepared using DNA polymerases. DNA nanoflowers combine the high surface area and scaffolding of inorganic Mg2P2O7 nanocrystals with the targeting properties of DNA, whilst adding enzymatic stability and enhanced cellular uptake. We have investigated conditions for chemically modifying the inorganic core of these nanoflowers through substitution of Mg2+ with Mn2+, Co2+ or Zn2+ and have characterised the resulting particles. These have a range of novel nanoarchitectures, retain the enzymatic stability of their magnesium counterparts and the Co2+ and Mn2+ DNA nanoflowers have added magnetic properties. We investigate conditions to control different morphologies, DNA content, hybridisation properties, and size. Additionally, we show that DNA nanoflower production is not limited to Ф29 DNA polymerase and that the choice of polymerase can influence the DNA length within the constructs. We anticipate that the added control of structure, size and chemistry will enhance future applications

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science > Physics
Library of Congress Subject Headings (LCSH): DNA polymerases, Nanocrystals, Nucleic acids
Journal or Publication Title: Nucleic Acids Research
Publisher: Oxford University Press
ISSN: 0305-1048
Official Date: 6 September 2018
Dates:
DateEvent
6 September 2018Published
12 July 2018Available
29 June 2018Accepted
Date of first compliant deposit: 27 June 2018
Volume: 46
Number: 15
Page Range: pp. 7495-7505
DOI: 10.1093/nar/gky630
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
RPG-2015-005Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
BB/J001694/2 [BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
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