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Structure and biocatalytic scope of coclaurine N-methyltransferase
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Bennett, Matthew R., Thompson, Mark L., Shepherd, Sarah A., Dunstan, Mark S., Herbert, Abigail J., Smith, Duncan R. M., Cronin, Victoria A., Menon, Binuraj R. K., Levy, Colin and Micklefield, Jason (2018) Structure and biocatalytic scope of coclaurine N-methyltransferase. Angewandte Chemie International Edition, 57 (33). pp. 10600-10604. doi:10.1002/anie.201805060 ISSN 1433-7851.
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Official URL: http://dx.doi.org/10.1002/anie.201805060
Abstract
Benzylisoquinoline alkaloids (BIAs) are a structurally diverse family of plant secondary metabolites, which have been exploited to develop analgesics, antibiotics, antitumor agents, and other therapeutic agents. Biosynthesis of BIAs proceeds via a common pathway from tyrosine to (S)‐reticulene at which point the pathway diverges. Coclaurine N‐methyltransferase (CNMT) is a key enzyme in the pathway to (S)‐reticulene, installing the N‐methyl substituent that is essential for the bioactivity of many BIAs. In this paper, we describe the first crystal structure of CNMT which, along with mutagenesis studies, defines the enzymes active site architecture. The specificity of CNMT was also explored with a range of natural and synthetic substrates as well as co‐factor analogues. Knowledge from this study could be used to generate improved CNMT variants required to produce BIAs or synthetic derivatives.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Angewandte Chemie International Edition | ||||||||
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | ||||||||
ISSN: | 1433-7851 | ||||||||
Official Date: | 13 August 2018 | ||||||||
Dates: |
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Volume: | 57 | ||||||||
Number: | 33 | ||||||||
Page Range: | pp. 10600-10604 | ||||||||
DOI: | 10.1002/anie.201805060 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) |
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