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Investigating cell uptake of guanidinium-rich RAFT polymers : impact of comonomer and monomer distribution
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Martin, Liam T., Peltier, Raoul, Kuroki, Agnès, Town, James and Perrier, Sébastien (2018) Investigating cell uptake of guanidinium-rich RAFT polymers : impact of comonomer and monomer distribution. Biomacromolecules, 19 (8). pp. 3190-3200. doi:10.1021/acs.biomac.8b00146 ISSN 1525-7797.
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WRAP-investigating-cell-uptake-guanidinium-rich-RAFT-polymers-Perrier-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1590Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.biomac.8b00146
Abstract
A range of well-defined guanidinium-rich linear polymers with demonstrable efficiency for cellular internalization were developed. A protected guanidinium-functional acrylamide monomer (di-Boc-guanidinium ethyl acrylamide, GEAdiBoc) was synthesized and then polymerized via RAFT polymerization to yield well-defined homopolymers, which were then deprotected and functionalized with a fluorescein dye to observe and quantify their cellular uptake. The cellular uptake of these homopolymers was first compared to analogous polyarginines, which are commonly used in modern drug delivery. Following this, a range of well-defined guanidinium-rich copolymers were prepared in which the monomer distribution was varied using a convenient one-pot sequential RAFT polymerization approach. Systematic quantification of the cell uptake of these compounds, supported by fluorescent confocal microscopy data, revealed that while the overall hydrophobicity of the resulting copolymers has a direct impact on the amount of copolymer taken up by cells, the distribution of monomers has an influence on both the extent of uptake and the relative extent to which each route of internalization (endocytosis vs direct translocation) is exploited.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QD Chemistry | |||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Drug delivery systems, Addition polymerization, Arginine, Pharmacokinetics, Copolymers, Fragmentation reactions | |||||||||||||||
Journal or Publication Title: | Biomacromolecules | |||||||||||||||
Publisher: | American Chemical Society | |||||||||||||||
ISSN: | 1525-7797 | |||||||||||||||
Official Date: | 13 August 2018 | |||||||||||||||
Dates: |
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Volume: | 19 | |||||||||||||||
Number: | 8 | |||||||||||||||
Page Range: | pp. 3190-3200 | |||||||||||||||
DOI: | 10.1021/acs.biomac.8b00146 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
Date of first compliant deposit: | 4 July 2018 | |||||||||||||||
Date of first compliant Open Access: | 11 June 2019 | |||||||||||||||
RIOXX Funder/Project Grant: |
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