Apoptosis in mouse fetal and neonatal oocytes during meiotic prophase one
Ghafari , Fataneh, Gutierrez , Carlos G. and Hartshorne, Geraldine M.. (2007) Apoptosis in mouse fetal and neonatal oocytes during meiotic prophase one. BMC Development Biology, Vol.7 . p. 87. ISSN 1471-213x
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Official URL: http://dx.doi.org/10.1186/1471-213X-7-87
The vast majority of oocytes formed in the fetal ovary do not survive beyond birth.
Possible reasons for their loss include the elimination of non-viable genetic
constitutions arising through meiosis, however, the precise relationship between
meiotic stages and prenatal apoptosis of oocytes remains elusive. We studied oocytes
in mouse fetal and neonatal ovaries, 14.5–21 days post coitum, to examine the
relationship between oocyte development and programmed cell death during meiotic
Microspreads of fetal and neonatal ovarian cells underwent immunocytochemistry for
meiosis- and apoptosis-related markers. COR-1 (meiosis-specific) highlighted axial
elements of the synaptonemal complex and allowed definitive identification of the
stages of meiotic prophase I. Labelling for cleaved poly-(ADP-ribose) polymerase
(PARP-1), an inactivated DNA repair protein, indicated apoptosis. The same oocytes
were then labelled for DNA double strand breaks (DSBs) using TUNEL. 1960
oocytes produced analysable results. .
Oocytes at all stages of meiotic prophase I stained for cleaved PARP-1 and/or TUNEL, or neither. Oocytes with fragmented (19.8%) or compressed (21.2%) axial
elements showed slight but significant differences in staining for cleaved PARP-1 and
TUNEL to those with intact elements. However, fragmentation of axial elements
alone was not a good indicator of cell demise. Cleaved PARP-1 and TUNEL staining
were not necessarily coincident, showing that TUNEL is not a reliable marker of apoptosis in oocytes.
Our data indicate that apoptosis can occur throughout meiotic prophase I in mouse
fetal and early postnatal oocytes, with greatest incidence at the diplotene stage.
Careful selection of appropriate markers for oocyte apoptosis is essential.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
|Divisions:||Faculty of Medicine > Warwick Medical School|
|Library of Congress Subject Headings (LCSH):||Oocytes, Mammalian, Apoptosis|
|Journal or Publication Title:||BMC Development Biology|
|Publisher:||Biomed Central Publisher|
|Official Date:||24 July 2007|
|Page Range:||p. 87|
|Access rights to Published version:||Open Access|
Version accepted by publisher (post-print, after peer review, before copy-editing).
1. Krakauer DC, Mira A: Mitochondria and germ-cell death. Nature 1999,
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