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Analysis of the promoter region of the Xenopus borealis N-Cadherin gene

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Webber, P. M. (1993) Analysis of the promoter region of the Xenopus borealis N-Cadherin gene. PhD thesis, University of Warwick.

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Official URL: http://webcat.warwick.ac.uk/record=b3182028~S15

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Abstract

A Xenopus borealis genomic library was screened with the 5'-end of the Xenopus laevis N-Cadherin cDNA (DETRICK et a/., 1990). Four groups of clones were isolated that differed in restriction-enzyme digestion patterns.

The sequencing of one of these clones, 3-9/4.8BS/pBS, has identified regions of DNA highly homologous to the X.laevis N-Cadherin gene. Accordingly, it is believed that the clone 3-9/4.8BS/pBS contains the 5'-end and promoter region of the X.boreal is N-Cadherin gene.

A sequence analysis of this region has shown it to be GC-rich and has revealed consensus TATA-box, CCAAT-box and Spl binding sites. Other possible transcription-factor binding-sites have also been identified, as well as the first exon/intron boundary.

A series of promoter-deletions were fused to the bacterial 3- galactosidase gene and micro-injected into X.laevis embryos. The 3- galactosidase staining patterns of whole embryos has visually shown that 1.3Kb of genomic DNA upstream of the translational start-site is sufficient to direct neural-specific transcription of this reporter gene.

Item Type: Thesis or Dissertation (PhD)
Subjects: Q Science > QH Natural history > QH426 Genetics
Library of Congress Subject Headings (LCSH): Xenopus -- Genetics, Cadherins, Promoters (Genetics), Sequence alignment (Bioinformatics)
Official Date: August 1993
Dates:
DateEvent
August 1993Submitted
Institution: University of Warwick
Theses Department: Department of Biological Sciences
Thesis Type: PhD
Publication Status: Unpublished
Sponsors: Medical Research Council (Great Britain)
Format of File: pdf
Extent: ix, 326 leaves : illustrations, charts
Language: eng

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