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FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy
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Clarke, Nicholas I. and Royle, Stephen J. (2018) FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy. Nature Communications, 9 (1). 2604. doi:10.1038/s41467-018-04993-0 ISSN 2041-1723.
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Official URL: http://dx.doi.org/10.1038/s41467-018-04993-0
Abstract
A current challenge is to develop tags to precisely visualize proteins in cells by light and electron microscopy. Here, we introduce FerriTag, a genetically-encoded chemically-inducible tag for correlative light-electron microscopy. FerriTag is a fluorescent recombinant electron-dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced heterodimerization. We demonstrate the utility of FerriTag for correlative light-electron microscopy by labeling proteins associated with various intracellular structures including mitochondria, plasma membrane, and clathrin-coated pits and vesicles. FerriTagging has a good signal-to-noise ratio and a labeling resolution of approximately 10 nm. We demonstrate how FerriTagging allows nanoscale mapping of protein location relative to a subcellular structure, and use it to detail the distribution and conformation of huntingtin-interacting protein 1 related (HIP1R) in and around clathrin-coated pits.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Electron microscopy, Proteins -- Affinity labeling, Cytology | |||||||||
Journal or Publication Title: | Nature Communications | |||||||||
Publisher: | Nature Publishing Group | |||||||||
ISSN: | 2041-1723 | |||||||||
Official Date: | 4 July 2018 | |||||||||
Dates: |
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Volume: | 9 | |||||||||
Number: | 1 | |||||||||
Article Number: | 2604 | |||||||||
DOI: | 10.1038/s41467-018-04993-0 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Date of first compliant deposit: | 10 July 2018 | |||||||||
Date of first compliant Open Access: | 10 July 2018 | |||||||||
RIOXX Funder/Project Grant: |
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