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CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis
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Schweisguth, François, Crespo-Yàñez, Xènia, Aguilar-Gurrieri, Carmen, Jacomin, Anne-Claire, Journet, Agnès, Mortier, Magda, Taillebourg, Emmanuel, Soleilhac, Emmanuelle, Weissenhorn, Winfried and Fauvarque, Marie-Odile (2018) CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis. PLoS Genetics, 14 (6). e1007456. doi:10.1371/journal.pgen.1007456 ISSN 1553-7390.
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Official URL: http://dx.doi.org/10.1371/journal.pgen.1007456
Abstract
Integration and down-regulation of cell growth and differentiation signals rely on plasma membrane receptor endocytosis and sorting towards either recycling vesicles or degradative lysosomes via multivesicular bodies (MVB). In this process, the endosomal sorting complex-III required for transport (ESCRT-III) controls membrane deformation and scission triggering intraluminal vesicle (ILV) formation at early endosomes. Here, we show that the ESCRT-III member CHMP1B can be ubiquitinated within a flexible loop known to undergo conformational changes during polymerization. We demonstrate further that CHMP1B is deubiquitinated by the ubiquitin specific protease USP8 (syn. UBPY) and found fully devoid of ubiquitin in a ~500 kDa large complex that also contains its ESCRT-III partner IST1. Moreover, EGF stimulation induces the rapid and transient accumulation of ubiquitinated forms of CHMP1B on cell membranes. Accordingly, CHMP1B ubiquitination is necessary for CHMP1B function in both EGF receptor trafficking in human cells and wing development in Drosophila. Based on these observations, we propose that CHMP1B is dynamically regulated by ubiquitination in response to EGF and that USP8 triggers CHMP1B deubiquitination possibly favoring its subsequent assembly into a membrane-associated ESCRT-III polymer.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QP Physiology | |||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Endocytosis, Ubiquitin, Cytology, Cells -- Growth | |||||||||||||||||||||
Journal or Publication Title: | PLoS Genetics | |||||||||||||||||||||
Publisher: | Public Library of Science | |||||||||||||||||||||
ISSN: | 1553-7390 | |||||||||||||||||||||
Official Date: | 22 June 2018 | |||||||||||||||||||||
Dates: |
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Volume: | 14 | |||||||||||||||||||||
Number: | 6 | |||||||||||||||||||||
Article Number: | e1007456 | |||||||||||||||||||||
DOI: | 10.1371/journal.pgen.1007456 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 13 July 2018 | |||||||||||||||||||||
Date of first compliant Open Access: | 16 July 2018 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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