Wang, Donghao, Ding, Wenyi, Zhou, Kaiyue, Guo, Shutong, Zhang, Qiang and Haddleton, David M. (2018) Coating titania nanoparticles with epoxy-containing catechol polymers via Cu(0)-living radical polymerization as intelligent enzyme carriers. Biomacromolecules, 19 (7). pp. 2979-2990. doi:10.1021/acs.biomac.8b00544

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Abstract

Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role in the enzyme’s industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper-mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA) directly from an unprotected dopamine-functionalized initiator to obtain an epoxy-containing polymer with terminal anchor for the “grafting to” or “one-pot” modification of titania nanoparticles. A rhodamine B-labeled laccase has been subsequently used as a model enzyme for successful immobilization to yield an intelligent titania/laccase hybrid bifunctional catalyst. The immobilized laccase has shown excellent thermal stability under ambient or even relatively high temperature above the lower critical solution temperature (LCST) at which temperature the hybrid particles could be facilely recovered for reuse. The enzyme activity could be maintained during the repeated use after recovery and enzymatic degradation of bisphenol A was proven to be efficient. The photocatalytic ability of titania was also investigated by fast degradation of rhodamine B under the excitation of simulated sunlight. Therefore, this study has provided a facile strategy for the immobilization of metal oxide catalysts with enzymes, which constructs a novel bifunctional catalyst that will be promising for the “one-pot” degradation of different organic pollutants.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Science > Chemistry
SWORD Depositor:	Library Publications Router
Library of Congress Subject Headings (LCSH):	Enzymes -- Industrial applications, Polymers, Nanoparticles, Titanium dioxide
Journal or Publication Title:	Biomacromolecules
Publisher:	American Chemical Society (ACS)
ISSN:	1526-4602
Official Date:	9 July 2018
Dates:	Date Event 9 July 2018 Published 8 May 2018 Available 8 May 2018 Accepted
Date of first compliant deposit:	29 November 2018
Volume:	19
Number:	7
Page Range:	pp. 2979-2990
DOI:	10.1021/acs.biomac.8b00544
Status:	Peer Reviewed
Publication Status:	Published
Publisher Statement:	“This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].”
Access rights to Published version:	Restricted or Subscription Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID 21504044 [NSFC] National Natural Science Foundation of China http://dx.doi.org/10.13039/501100001809 BK20150769 Natural Science Foundation of Jiangsu Province http://dx.doi.org/10.13039/501100004608 30916011203 [MEPRC] Ministry of Education of the People's Republic of China http://dx.doi.org/10.13039/501100002338 157453 China Postdoctoral Science Foundation http://dx.doi.org/10.13039/501100002858

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