The Library
Human coronavirus NL63 molecular epidemiology and evolutionary patterns in rural coastal Kenya
Tools
Tools
Tools
Kiyuka, Patience K, Agoti, Charles N, Munywoki, Patrick K, Njeru, Regina, Bett, Anne, Otieno, James R, Otieno, Grieven P, Kamau, Everlyn, Clark, Taane G, van der Hoek, Lia, Kellam, Paul, Nokes, D. James and Cotten, Matthew (2018) Human coronavirus NL63 molecular epidemiology and evolutionary patterns in rural coastal Kenya. The Journal of Infectious Diseases, 217 (11). pp. 1728-1739. doi:10.1093/infdis/jiy098 ISSN 0022-1899.
|
PDF
WRAP-human-coronavirus-NL63-molecular-epidemiology-evolutionary-patterns-Nokes-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1187Kb) | Preview |
Official URL: http://dx.doi.org/10.1093/infdis/jiy098
Abstract
Background:
Human coronavirus NL63 (HCoV-NL63) is a globally endemic pathogen causing mild and severe respiratory tract infections with reinfections occurring repeatedly throughout a lifetime.
Methods:
Nasal samples were collected in coastal Kenya through community-based and hospital-based surveillance. HCoV-NL63 was detected with multiplex real-time reverse transcription PCR, and positive samples were targeted for nucleotide sequencing of the spike (S) protein. Additionally, paired samples from 25 individuals with evidence of repeat HCoV-NL63 infection were selected for whole-genome virus sequencing.
Results:
HCoV-NL63 was detected in 1.3% (75/5573) of child pneumonia admissions. Two HCoV-NL63 genotypes circulated in Kilifi between 2008 and 2014. Full genome sequences formed a monophyletic clade closely related to contemporary HCoV-NL63 from other global locations. An unexpected pattern of repeat infections was observed with some individuals showing higher viral titers during their second infection. Similar patterns for 2 other endemic coronaviruses, HCoV-229E and HCoV-OC43, were observed. Repeat infections by HCoV-NL63 were not accompanied by detectable genotype switching.
Conclusions:
In this coastal Kenya setting, HCoV-NL63 exhibited low prevalence in hospital pediatric pneumonia admissions. Clade persistence with low genetic diversity suggest limited immune selection, and absence of detectable clade switching in reinfections indicates initial exposure was insufficient to elicit a protective immune response.
| Item Type: | Journal Article | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | R Medicine > RC Internal medicine R Medicine > RJ Pediatrics > RJ101 Child Health. Child health services |
|||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Coronavirus infections -- Kenya, Respiratory infections -- Kenya, Pneumonia in children -- Kenya, Medicine, Rural -- Kenya | |||||||||||||||||||||
| Journal or Publication Title: | The Journal of Infectious Diseases | |||||||||||||||||||||
| Publisher: | Oxford University Press | |||||||||||||||||||||
| ISSN: | 0022-1899 | |||||||||||||||||||||
| Official Date: | 5 May 2018 | |||||||||||||||||||||
| Dates: |
|
|||||||||||||||||||||
| Volume: | 217 | |||||||||||||||||||||
| Number: | 11 | |||||||||||||||||||||
| Page Range: | pp. 1728-1739 | |||||||||||||||||||||
| DOI: | 10.1093/infdis/jiy098 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 17 July 2018 | |||||||||||||||||||||
| Date of first compliant Open Access: | 17 July 2018 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
