UNSPECIFIED. (2002) Molecular analysis of a bacterial chitinolytic community in an upland pasture. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68 (10). pp. 5042-5050. ISSN 0099-2240

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Abstract

The effects of agricultural-improvement treatments on the chitinolytic activity and diversity of a microbial community were investigated within an upland pasture. The treatments of interest were lime and treated sewage sludge, both commonly applied to pasture land to improve fertility. Burial of chitin-containing litter bags at the field site resulted in enrichment of bacteria according to 16S rRNA fingerprinting. Chitinolytic-activity measurements showed that the highest activity occurred in those bags recovered from sludge-amended plots, which correlated well with increased counts of actinobacteria in samples from these chitin bags. Our findings suggest that sewage sludge increases the fertility of the soil in terms of chitinase activity. Ten clone libraries were constructed from family 18 subgroup A chitinases, PCR amplified from litter bags buried in soil in July 2000 or in September 2000, in a separate study. Analysis of these libraries by restriction fragment length polymorphism and sequencing showed that they were dominated by actinobacterium-like chitinase sequences. This suggests that actinobacteria have an important chitinolytic function in this soil ecosystem. Our findings showed that sludge application increased chitinolytic activity but decreased the diversity of chitinases present.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0099-2240
Date:	October 2002
Volume:	68
Number:	10
Number of Pages:	9
Page Range:	pp. 5042-5050
Identification Number:	10.1128/AEM.68.10.5042-5050.2002
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/10527

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