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Characterization of a human immunodeficiency virus type 1 pre-integration complex in which the majority of the cDNA is resistant to DNase I digestion

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UNSPECIFIED (2002) Characterization of a human immunodeficiency virus type 1 pre-integration complex in which the majority of the cDNA is resistant to DNase I digestion. JOURNAL OF GENERAL VIROLOGY, 83 (Part 10). pp. 2523-2532. ISSN 0022-1317.

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Abstract

The human immunodeficiency virus type 1 (HIV-1) pre-integration complex (PIC) is a cytoplasmic nucleoprotein structure derived from the core of the virion and is responsible for reverse transcription of viral RNA to cDNA, transport to the nucleus and integration of the cDNA into the genome of the infected target cell. Others have shown by Mu phage-mediated PCR footprinting that only the LTRs of the cDNA of PICs isolated early in infection are protected by bound protein, while the rest of the genome is susceptible to nuclease attack. Here, using DNase I footprinting, we confirmed that the majority of the cDNA of PICs isolated at 8.5 h after infection with cell-free virus was sensitive to digestion with DNase I and that only part of the LTRs (approximately 6% of the total cDNA) was protected. However, PICs isolated 90 min later (at 10 h post-infection) were very different in that the majority (approximately 90%) of cDNA was protected from nuclease degradation. These late PICs were integration active in vitro. We conclude that HIV-1 has at least two types of PIC, an early PIC characterized by protein bound only at the LTRs, and a late, and possibly more mature form, in which protein is bound along the length of the cDNA.

Item Type: Journal Article
Subjects: T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title: JOURNAL OF GENERAL VIROLOGY
Publisher: SOC GENERAL MICROBIOLOGY
ISSN: 0022-1317
Official Date: October 2002
Dates:
DateEvent
October 2002UNSPECIFIED
Volume: 83
Number: Part 10
Number of Pages: 10
Page Range: pp. 2523-2532
Publication Status: Published

Data sourced from Thomson Reuters' Web of Knowledge

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