Pinheiro, Philip, Scarlett, Garry, Rodger, Alison, Rodger, P. Mark, Murray, Anna, Brown, Tom, Newbury, Sarah F. and McCellan, James A.. (2002) Structures of CUG repeats in RNA - Potential implications for human genetic diseases. Journal of Biological Chemistry, 277 (38). pp. 35183-35190. ISSN 0021-9258

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Abstract

Triplet repeats that cause human genetic diseases have been shown to exhibit unusual compact structures in DNA, and in this paper we show that similar structures exist in shorter "normal length" CNG RNA. CUG and control RNAs were made chemically and by in vitro transcription. We find that "normal" short CUG RNAs migrate anomalously fast on non-denaturing gels, compared with control oligos of similar base composition. By contrast, longer tracts approaching clinically relevant lengths appear to form higher order structures. The CD spectrum of shorter tracts is similar to triplex and pseudoknot nucleic acid structures and different from classical hairpin spectra. A model is outlined that enables the base stacking features of poly(r(G-C))(2).poly(r(U)) or poly(d(G-C))(2).poly(d(T)) triplexes to be achieved, even by a single 15-mer.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Journal of Biological Chemistry
Publisher:	American Society Biochemistry, Molecular Biology Inc.
ISSN:	0021-9258
Date:	20 September 2002
Volume:	277
Number:	38
Number of Pages:	8
Page Range:	pp. 35183-35190
Identification Number:	10.1074/jbc.M202235200
Status:	Peer Reviewed
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/10546

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