The Library
Deltaproteobacteria (Pelobacter) and Methanococcoides are responsible for choline-dependent methanogenesis in a coastal saltmarsh sediment
Tools
Jameson, Eleanor, Stephenson, Jason, Jones, Helen J., Millard, Andrew D., Kaster, K., Purdy, Kevin J., Airs, R., Murrell, J. C. (J. Colin) and Chen, Yin (2019) Deltaproteobacteria (Pelobacter) and Methanococcoides are responsible for choline-dependent methanogenesis in a coastal saltmarsh sediment. ISME Journal, 13 (2). pp. 277-289. doi:10.1038/s41396-018-0269-8 ISSN 1751-7362.
|
PDF
WRAP-Deltaproteobacteria-choline-dependent-coastal-saltmarsh-Purdy-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1206Kb) | Preview |
|
PDF (Revised manuscript)
WRAP-deltaproteobacteria-pelobacter-methanococcoides-responsible-choline-coastal-Jameson-2018.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (560Kb) |
||
PDF
WRAP-deltaproteobacteria-pelobacter-methanococcoides-responsible-choline-dependent-methanogenesis-Chen-2018.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (1007Kb) |
Official URL: https://doi.org/10.1038/s41396-018-0269-8
Abstract
Coastal saltmarsh sediments represent an important source of natural methane emissions, much of which originates from quaternary and methylated amines, such as choline and trimethylamine. In this study, we combine DNA stable isotope probing with high throughput sequencing of 16S rRNA genes and 13C2-choline enriched metagenomes, followed by metagenome data assembly, to identify the key microbes responsible for methanogenesis from choline. Microcosm incubation with 13C2-choline leads to the formation of trimethylamine and subsequent methane production, suggesting that choline-dependent methanogenesis is a two-step process involving trimethylamine as the key intermediate. Amplicon sequencing analysis identifies Deltaproteobacteria of the genera Pelobacter as the major choline utilizers. Methanogenic Archaea of the genera Methanococcoides become enriched in choline-amended microcosms, indicating their role in methane formation from trimethylamine. The binning of metagenomic DNA results in the identification of bins classified as Pelobacter and Methanococcoides. Analyses of these bins reveal that Pelobacter have the genetic potential to degrade choline to trimethylamine using the choline-trimethylamine lyase pathway, whereas Methanococcoides are capable of methanogenesis using the pyrrolysine-containing trimethylamine methyltransferase pathway. Together, our data provide a new insight on the diversity of choline utilizing organisms in coastal sediments and support a syntrophic relationship between Bacteria and Archaea as the dominant route for methanogenesis from choline in this environment.
Item Type: | Journal Article | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | |||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
|||||||||||||||
Library of Congress Subject Headings (LCSH): | Salt marshes, Choline, Atmospheric methane, Methane, Methanobacteriaceae, Methanotrophs | |||||||||||||||
Journal or Publication Title: | ISME Journal | |||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||
ISSN: | 1751-7362 | |||||||||||||||
Official Date: | February 2019 | |||||||||||||||
Dates: |
|
|||||||||||||||
Volume: | 13 | |||||||||||||||
Number: | 2 | |||||||||||||||
Page Range: | pp. 277-289 | |||||||||||||||
DOI: | 10.1038/s41396-018-0269-8 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 27 July 2018 | |||||||||||||||
Date of first compliant Open Access: | 14 September 2018 | |||||||||||||||
Funder: | This work was supported by the Natural Environment Research Council (NERC), UK, through research grants (NE/I027061/1 and NE/I025077/1) and a Ph.D. studentship to H.J. and a Warwick Integrative Synthetic Biology (WISB) early career fellowship, funded join | |||||||||||||||
RIOXX Funder/Project Grant: |
|
|||||||||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year