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Microfluidic deposition for resolving single molecule protein architecture and heterogeneity
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Ruggeri, Francesco Simone, Charmet, Jérôme, Kartanas, Tadas, Peter, Quentin, Chia, Sean, Habchi, Johnny, Dobson, Christopher M., Vendruscolo, Michele and Knowles, Tuomas P. J. (2018) Microfluidic deposition for resolving single molecule protein architecture and heterogeneity. Nature Communications, 9 . 3890. doi:10.1038/s41467-018-06345-4
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Official URL: https://doi.org/10.1038/s41467-018-06345-4
Abstract
Scanning probe microscopy provides a unique window into the morphology, mechanics and structure of proteins and their complexes on the nanoscale. Such measurements require, however, deposition of samples onto substrates. This process can affect conformations and assembly states of the molecular species under investigation and can bias the molecular populations observed in heterogeneous samples through differential adsorption. Here, we show that these limitations can be overcome with a single-step microfluidic spray deposition platform. This method transfers biological solutions to substrates as microdroplets with sub-picoliter volume, drying in milliseconds, a timescale that is shorter than typical diffusion times of proteins on liquid-solid interfaces, thus avoiding surface mass transport and change to the assembly state. Finally, the single-step deposition ensures the attachment of the full molecular content of the sample to the substrate, allowing quantitative measurements of different molecular populations within heterogeneous systems, including protein aggregates.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history |
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| Divisions: | Faculty of Science > WMG (Formerly the Warwick Manufacturing Group) | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Proteins -- Molecular aspects, Nanochemistry, Scanning probe microscopy, Mass transport (Physics) | ||||||||||||||||||
| Journal or Publication Title: | Nature Communications | ||||||||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||||||||
| ISSN: | 2041-1723 | ||||||||||||||||||
| Official Date: | 24 September 2018 | ||||||||||||||||||
| Dates: |
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| Volume: | 9 | ||||||||||||||||||
| Article Number: | 3890 | ||||||||||||||||||
| DOI: | 10.1038/s41467-018-06345-4 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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