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Microfluidic deposition for resolving single molecule protein architecture and heterogeneity

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Ruggeri, Francesco Simone, Charmet, Jérôme, Kartanas, Tadas, Peter, Quentin, Chia, Sean, Habchi, Johnny, Dobson, Christopher M., Vendruscolo, Michele and Knowles, Tuomas P. J. (2018) Microfluidic deposition for resolving single molecule protein architecture and heterogeneity. Nature Communications, 9 . 3890. doi:10.1038/s41467-018-06345-4

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Official URL: https://doi.org/10.1038/s41467-018-06345-4

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Abstract

Scanning probe microscopy provides a unique window into the morphology, mechanics and structure of proteins and their complexes on the nanoscale. Such measurements require, however, deposition of samples onto substrates. This process can affect conformations and assembly states of the molecular species under investigation and can bias the molecular populations observed in heterogeneous samples through differential adsorption. Here, we show that these limitations can be overcome with a single-step microfluidic spray deposition platform. This method transfers biological solutions to substrates as microdroplets with sub-picoliter volume, drying in milliseconds, a timescale that is shorter than typical diffusion times of proteins on liquid-solid interfaces, thus avoiding surface mass transport and change to the assembly state. Finally, the single-step deposition ensures the attachment of the full molecular content of the sample to the substrate, allowing quantitative measurements of different molecular populations within heterogeneous systems, including protein aggregates.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
Divisions: Faculty of Science > WMG (Formerly the Warwick Manufacturing Group)
Library of Congress Subject Headings (LCSH): Proteins -- Molecular aspects, Nanochemistry, Scanning probe microscopy, Mass transport (Physics)
Journal or Publication Title: Nature Communications
Publisher: Nature Publishing Group
ISSN: 2041-1723
Official Date: 24 September 2018
Dates:
DateEvent
24 September 2018Published
31 July 2018Accepted
Volume: 9
Article Number: 3890
DOI: 10.1038/s41467-018-06345-4
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
P2ELP2_162116[SNSF] Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschunghttp://dx.doi.org/10.13039/501100001711
P300P2_171219[SNSF] Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschunghttp://dx.doi.org/10.13039/501100001711
UNSPECIFIEDDarwin College, University of Cambridgehttp://dx.doi.org/10.13039/501100000595
337969European Research Councilhttp://viaf.org/viaf/130022607
74979-NANOTRANSH2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
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