The Library
Combining affinity selection and specific ion mobility for microchip protein sensing
Tools
Arter, William E., Charmet, Jérôme, Kong, Jinglin, Saar, Kadi L., Herling, Therese W., Müller, Thomas, Keyser, Ulrich F. and Knowles, Tuomas P. J. (2018) Combining affinity selection and specific ion mobility for microchip protein sensing. Analytical Chemistry, 90 (17). pp. 10302-10310. doi:10.1021/acs.analchem.8b02051 ISSN 0003-2700.
|
PDF
WRAP-combining-affinity-selection-specific-ion-mobility-microchip-protein-sensing-Charmet-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1340Kb) | Preview |
Official URL: https://doi.org/10.1021/acs.analchem.8b02051
Abstract
The sensitive detection of proteins is a key objective in many areas of biomolecular science, ranging from biophysics to diagnostics. However, sensing in complex biological fluids is hindered by non-specific interactions with off-target species. Here, we describe and demonstrate an assay that utilises both the chemical and physical properties of the target species to achieve high selectivity, in a manner not possible by chemical complementarity alone, in complex media. We achieve this objective through a combinatorial strategy, by simultaneously exploiting free-flow electrophoresis for target selection, on the basis of electrophoretic mobility, and conventional affinity-based selection. In addition, we demonstrate amplification of the resultant signal by a catalytic DNA nano-circuit. This approach brings together the inherent solution-phase advantages of microfluidic sample handling with isothermal, enzyme-free signal amplification. With this method, no surface immobilisation or washing steps are required and our assay is well suited to mono-epitopic targets, presenting advantages over conventional ELISA techniques.
Item Type: | Journal Article | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Biomolecules, Proteins, Integrated circuits | ||||||||||||||||||
Journal or Publication Title: | Analytical Chemistry | ||||||||||||||||||
Publisher: | American Chemical Society | ||||||||||||||||||
ISSN: | 0003-2700 | ||||||||||||||||||
Official Date: | 4 September 2018 | ||||||||||||||||||
Dates: |
|
||||||||||||||||||
Volume: | 90 | ||||||||||||||||||
Number: | 17 | ||||||||||||||||||
Page Range: | pp. 10302-10310 | ||||||||||||||||||
DOI: | 10.1021/acs.analchem.8b02051 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Reuse Statement (publisher, data, author rights): | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.8b02051 | ||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
Date of first compliant deposit: | 6 August 2018 | ||||||||||||||||||
Date of first compliant Open Access: | 2 August 2019 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year