Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Photoinitiated polymerization-induced self-assembly in the presence of surfactants enables membrane protein incorporation into vesicles

Tools
- Tools
+ Tools

Varlas, Spyridon, Blackman, Lewis D., Findlay, Heather E., Reading, Eamonn, Booth, Paula J., Gibson, Matthew I. and O’Reilly, Rachel K. (2018) Photoinitiated polymerization-induced self-assembly in the presence of surfactants enables membrane protein incorporation into vesicles. Macromolecules, 51 (16). pp. 6190-6201. doi:10.1021/acs.macromol.8b00994

Research output not available from this repository, contact author.
Official URL: https://dx.doi.org/10.1021/acs.macromol.8b00994

Request Changes to record.

Abstract

Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations and under mild reaction conditions, it has the potential to have significant industrial scope. However, given that the majority of industrial (and more specifically biotechnological) formulations contain mixtures of polymers and surfactants, the effect of such surfactants on the PISA process is an important consideration. Thus, to expand the scope of the methodology, the effect of small molecule surfactants on the PISA process, specifically for the preparation of unilamellar vesicles, was investigated. Similar to aqueous photo-PISA findings in the absence of surfactant molecules, the originally targeted vesicular morphology was retained in the presence of varying concentrations of non-ionic surfactants, while a diverse set of lower-order morphologies was observed for ionic surfactants. Interestingly, a critical micelle concentration (CMC)-dependent behavior was detected in the case of zwitterionic detergents. Additionally, tunable size and membrane thickness of vesicles were observed by using different types and concentration of surfactants. Based on these findings, a functional channel-forming membrane protein (OmpF porin), stabilized in aqueous media by surfactant molecules, was able to be directly inserted into the membrane of vesicles during photo-PISA. Our study demonstrates the potential of photo-PISA for the direct formation of protein–polymer complexes and highlights how this method could be used to design biomimicking polymer/surfactant nanoreactors.

Item Type: Journal Article
Divisions: Faculty of Science > Chemistry
Journal or Publication Title: Macromolecules
Publisher: American Chemical Society
ISSN: 0024-9297
Official Date: 2018
Dates:
DateEvent
2018Published
6 August 2018Available
Volume: 51
Number: 16
Page Range: pp. 6190-6201
DOI: 10.1021/acs.macromol.8b00994
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us