Steroids mediate the expression of cytoplasmic and membrane-linked components in human myometrial cells
UNSPECIFIED. (2002) Steroids mediate the expression of cytoplasmic and membrane-linked components in human myometrial cells. MOLECULAR HUMAN REPRODUCTION, 8 (7). pp. 597-605. ISSN 1360-9947Full text not available from this repository.
It is well known that the smooth muscle of the human myometrium is a target for the steroid hormones progesterone (P-4) and estrogen. Progesterone is believed to participate in the maintenance of pregnancy, while estrogen is possibly involved in the process of parturition by promoting cervical dilatation. We examined the combined effects of P-4 and 17beta-estradiol (E-2) on components of signalling pathways in human myometrial cells in vitro by immunoblotting. Long-term treatment of myometrial cells with a series of concentrations of P-4 and E-2 in combination caused a change in the phosphorylation status of P42/44 mitogen-activated protein kinase and of c-Jun N-terminal kinase (SAPK/JNK). P-4 and E-2 caused a decrease in protein expression of Gqalpha, Gzalpha, Gi(1/2)alpha and, to a lesser extent, G(0)alpha. The two steroids caused a decrease in the expression of the two small GSalpha isoforms. Cyclo-oxygenase-2 expression was increased by 2.5-fold after steroid treatment, while proliferating cell nuclear antigen expression levels remained unchanged. These observations show that the combination of P-4 and E-2 influences intracellular and membrane-bound components of signal transduction pathways in human myometrial cells. The implications of the two steroid hormones on intracellular signalling pathways in the human myometrium merits further investigation.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
R Medicine > RG Gynecology and obstetrics
|Journal or Publication Title:||MOLECULAR HUMAN REPRODUCTION|
|Publisher:||OXFORD UNIV PRESS|
|Number of Pages:||9|
|Page Range:||pp. 597-605|
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