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Application of tandem two-dimensional mass spectrometry for top-down deep sequencing of calmodulin
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Floris, Federico, Chiron, Lionel, Lynch, Alice M., Barrow, Mark P., Delsuc, Marc-André and O’Connor, Peter B. (2018) Application of tandem two-dimensional mass spectrometry for top-down deep sequencing of calmodulin. Journal of The American Society for Mass Spectrometry, 29 (8). pp. 1700-1705. doi:10.1007/s13361-018-1978-y ISSN 1044-0305.
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WRAP-application-tandem-two-dimensional-mass-spectrometry-O'Connor-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1292Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s13361-018-1978-y
Abstract
Two-dimensional mass spectrometry (2DMS) involves simultaneous acquisition of the fragmentation patterns of all the analytes in a mixture by correlating their precursor and fragment ions by modulating precursor ions systematically through a fragmentation zone. Tandem two-dimensional mass spectrometry (MS/2DMS) unites the ultra-high accuracy of Fourier transform ion cyclotron resonance (FT-ICR) MS/MS and the simultaneous data-independent fragmentation of 2DMS to achieve extensive inter-residue fragmentation of entire proteins. 2DMS was recently developed for top-down proteomics (TDP), and applied to the analysis of calmodulin (CaM), reporting a cleavage coverage of about ~23% using infrared multiphoton dissociation (IRMPD) as fragmentation technique. The goal of this work is to expand the utility of top-down protein analysis using MS/2DMS in order to extend the cleavage coverage in top-down proteomics further into the interior regions of the protein. In this case, using MS/2DMS, the cleavage coverage of CaM increased from ~23% to ~42%.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Calmodulin, Proteomics, Mass spectrometry | |||||||||||||||
| Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | |||||||||||||||
| Publisher: | Springer New York LLC | |||||||||||||||
| ISSN: | 1044-0305 | |||||||||||||||
| Official Date: | August 2018 | |||||||||||||||
| Dates: |
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| Volume: | 29 | |||||||||||||||
| Number: | 8 | |||||||||||||||
| Page Range: | pp. 1700-1705 | |||||||||||||||
| DOI: | 10.1007/s13361-018-1978-y | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 17 September 2018 | |||||||||||||||
| Date of first compliant Open Access: | 17 September 2018 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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