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Origins left, right, and centre : increasing the number of initiation sites in the escherichia coli chromosome
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Dimude, Juachi U., Stein, Monja, Andrzejewska, Ewa E., Khalifa, Mohammad S., Gajdosova, Alexandra, Retkute, Renata, Skovgaard, Ole and Rudolph, Christian J. (2018) Origins left, right, and centre : increasing the number of initiation sites in the escherichia coli chromosome. Genes, 9 (8). 376. doi:10.3390/genes9080376 ISSN 2073-4425.
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Official URL: http://dx.doi.org/10.3390/genes9080376
Abstract
The bacterium Escherichia coli contains a single circular chromosome with a defined architecture. DNA replication initiates at a single origin called oriC. Two replication forks are assembled and proceed in opposite directions until they fuse in a specialised zone opposite the origin. This termination area is flanked by polar replication fork pause sites that allow forks to enter, but not to leave. Thus, the chromosome is divided into two replichores, each replicated by a single replication fork. Recently, we analysed the replication parameters in E. coli cells, in which an ectopic origin termed oriZ was integrated in the right-hand replichore. Two major obstacles to replication were identified: (1) head-on replication–transcription conflicts at highly transcribed rrn operons, and (2) the replication fork trap. Here, we describe replication parameters in cells with ectopic origins, termed oriX and oriY, integrated into the left-hand replichore, and a triple origin construct with oriX integrated in the left-hand and oriZ in the right-hand replichore. Our data again highlight both replication–transcription conflicts and the replication fork trap as important obstacles to DNA replication, and we describe a number of spontaneous large genomic rearrangements which successfully alleviate some of the problems arising from having an additional origin in an ectopic location. However, our data reveal additional factors that impact efficient chromosome duplication, highlighting the complexity of chromosomal architecture.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | DNA replication, Escherichia coli, Bacterial chromosomes, Chromosomes -- Analysis | |||||||||
Journal or Publication Title: | Genes | |||||||||
Publisher: | M D P I AG | |||||||||
ISSN: | 2073-4425 | |||||||||
Official Date: | 27 July 2018 | |||||||||
Dates: |
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Volume: | 9 | |||||||||
Number: | 8 | |||||||||
Article Number: | 376 | |||||||||
DOI: | 10.3390/genes9080376 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 20 September 2018 | |||||||||
Date of first compliant Open Access: | 20 September 2018 | |||||||||
RIOXX Funder/Project Grant: |
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