The Library
Metallome of cerebrovascular endothelial cells infected with Toxoplasma gondii using μ-XRF imaging and inductively coupled plasma mass spectrometry
Tools
Tools
Tools
Al-Sandaqchi, Alaa T., Brignell, Chris, Collingwood, Joanna F., Geraki, Kalotina, Mirkes, Evgeny M., Kong, Kenny, Castellanos, Marcos, May, Sean T., Stevenson, Carl W. and Elsheikha, Hany M. (2018) Metallome of cerebrovascular endothelial cells infected with Toxoplasma gondii using μ-XRF imaging and inductively coupled plasma mass spectrometry. Metallomics, 10 (10). pp. 1401-1414. doi:10.1039/c8mt00136g ISSN 1756-5901.
|
PDF
c8mt00136g.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (5Mb) | Preview |
|
|
PDF
WRAP-metallome-cerebrovascular-endothelial-Toxoplasma-gondii-XRF-spectrometry-Collingwood-2018.pdf - Accepted Version - Requires a PDF viewer. Download (5Mb) | Preview |
Official URL: https://doi.org/10.1039/C8MT00136G
Abstract
In this study, we measured the levels of elements in human brain microvascular endothelial cells (ECs) infected with T. gondii. ECs were infected with tachyzoites of the RH strain, and at 6, 24, and 48 hours post infection (hpi), the intracellular concentrations of elements were determined using a synchrotron-microfocus X-ray fluorescence microscopy (μ-XRF) system. This method enabled the quantification of the concentrations of Zn and Ca in infected and uninfected (control) ECs at sub-micron spatial resolution. T. gondii-hosting ECs contained less Zn than uninfected cells only at 48 hpi (p < 0.01). The level of Ca was not significantly different between infected and control cells (p > 0.05). Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis revealed infection-specific metallome profiles characterized by significant increases in the intracellular levels of Zn, Fe, Mn and Cu at 48 hpi (p < 0.01), and significant reductions in the extracellular concentrations of Co, Cu, Mo, V, and Ag at 24 hpi (p < 0.05) compared with control cells. Zn constituted the largest part (74%) of the total metal composition (metallome) of the parasite. Gene expression analysis showed infection-specific upregulation in the expression of five genes, MT1JP, MT1M, MT1E, MT1F, and MT1X, belonging to the metallothionein gene family. These results point to a possible correlation between T. gondii infection and increased expression of MT1 isoforms and altered intracellular levels of elements, especially Zn and Fe. Taken together, a combined μ-XRF and ICP-MS approach is promising for studies of the role of elements in mediating host-parasite interaction.
| Item Type: | Journal Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QL Zoology Q Science > QP Physiology |
|||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Endothelial cells, Toxoplasma gondii -- Infections | |||||||||
| Journal or Publication Title: | Metallomics | |||||||||
| Publisher: | Royal Society of Chemistry | |||||||||
| ISSN: | 1756-5901 | |||||||||
| Official Date: | 17 October 2018 | |||||||||
| Dates: |
|
|||||||||
| Volume: | 10 | |||||||||
| Number: | 10 | |||||||||
| Page Range: | pp. 1401-1414 | |||||||||
| DOI: | 10.1039/c8mt00136g | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 10 October 2018 | |||||||||
| Date of first compliant Open Access: | 5 September 2019 | |||||||||
| RIOXX Funder/Project Grant: |
|
|||||||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
