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Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates
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UNSPECIFIED. (2002) Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates. BIOCATALYSIS AND BIOTRANSFORMATION, 20 (4). pp. 257-264. ISSN 1024-2422
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Official URL: http://dx.doi.org/10.1080/10242420290029454
Abstract
The enantiopure (1S, 2S)-cis-dihydrodiol metabolites 2B-5B have been obtained in low yield from the corresponding monosubstituted halobenzene substrates 2A-5A, using a wild-type strain of Pseudomonas putida (ML2) containing benzene dioxygenase (BDO). Benzene cis-dihydrodiol dehydrogenase (BCD) from P. putida ML2 and naphthalene cis-dihydrodiol dehydrogenase (NCD) from P. putida 8859 were purified and used in a comparative study of the stereoselective biotransformation of cis-dihydrodiol enantiomers 2B-5B. The BCD and NCD enzymes were found to accept cis-dihydrodiol enantiomers of monosubstituted benzene cis-dihydrodiol substrates 2B-5B of opposite absolute configuration. The acyclic alkene 1,2-diols 10-17 were also found to be acceptable substrates for BCD.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
| Journal or Publication Title: | BIOCATALYSIS AND BIOTRANSFORMATION |
| Publisher: | TAYLOR & FRANCIS LTD |
| ISSN: | 1024-2422 |
| Date: | August 2002 |
| Volume: | 20 |
| Number: | 4 |
| Number of Pages: | 8 |
| Page Range: | pp. 257-264 |
| Identification Number: | 10.1080/10242420290029454 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/10880 |
Data sourced from Thomson Reuters' Web of Knowledge
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