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Boolean computation in plants using post-translational genetic control and a visual output signal
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Cordero, Teresa, Rosado, Arantxa, Majer, Eszter, Jaramillo, Alfonso, Rodrigo, Guillermo and Daròs, José-Antonio (2018) Boolean computation in plants using post-translational genetic control and a visual output signal. ACS Synthetic Biology, 7 (10). 2322–-2330. doi:10.1021/acssynbio.8b00214 ISSN 2161-5063.
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WRAP-Boolean-plants-post-translational-genetic-output-signal-Jaramillo-2018.pdf - Accepted Version - Requires a PDF viewer. Download (2644Kb) | Preview |
Official URL: https://doi.org/10.1021/acssynbio.8b00214
Abstract
Due to autotrophic growing capacity and extremely rich secondary metabolism, plants should be preferred targets of synthetic biology. However, developments in plants usually run below those in other taxonomic groups. In this work we engineered genetic circuits capable of logic YES, OR and AND Boolean computation in plant tissues with a visual output signal. The circuits, which are deployed by means of Agrobacterium tumefaciens, perform with the conditional activity of the MYB transcription factor Rosea1 from Antirrhinum majus inducing the accumulation of anthocyanins, plant endogenous pigments that are directly visible to the naked eye or accurately quantifiable by spectrophotometric analysis. The translational fusion of Rosea1 to several viral proteins, such as potyvirus NIb or fragments thereof, rendered the transcription factor inactive. However, anthocyanin accumulation could be restored by inserting protease cleavage sites between both moieties of the fusion and by coexpressing specific proteases, such as potyvirus nuclear inclusion a protease.
| Item Type: | Journal Article | |||||||||||||||||||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
| Journal or Publication Title: | ACS Synthetic Biology | |||||||||||||||||||||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||||||||||||||||||||
| ISSN: | 2161-5063 | |||||||||||||||||||||||||||
| Official Date: | 19 October 2018 | |||||||||||||||||||||||||||
| Dates: |
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| Volume: | 7 | |||||||||||||||||||||||||||
| Number: | 10 | |||||||||||||||||||||||||||
| Page Range: | 2322–-2330 | |||||||||||||||||||||||||||
| DOI: | 10.1021/acssynbio.8b00214 | |||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
| Date of first compliant deposit: | 9 October 2018 | |||||||||||||||||||||||||||
| Date of first compliant Open Access: | 13 September 2019 | |||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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