The Library
Genome-wide analysis of chromosomal import patterns after natural transformation of Helicobacter pylori
Tools
Bubendorfer, Sebastian, Krebes, Juliane, Yang, Ines, Hage, Elias, Schulz, Thomas F., Bahlawane, Christelle, Didelot, Xavier and Suerbaum, Sebastian (2016) Genome-wide analysis of chromosomal import patterns after natural transformation of Helicobacter pylori. Nature Communications, 7 . 11995. doi:10.1038/ncomms11995 ISSN 2041-1723.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1038/ncomms11995
Abstract
Recombination plays a dominant role in the evolution of the bacterial pathogen Helicobacter pylori, but its dynamics remain incompletely understood. Here we use an in vitro transformation system combined with genome sequencing to study chromosomal integration patterns after natural transformation. A single transformation cycle results in up to 21 imports, and repeated transformations generate a maximum of 92 imports (8% sequence replacement). Import lengths show a bimodal distribution with averages of 28 and 1,645 bp. Reanalysis of paired H. pylori genomes from chronically infected people demonstrates the same bimodal import pattern in vivo. Restriction endonucleases (REases) of the recipient bacteria fail to inhibit integration of homeologous DNA, independently of methylation. In contrast, REases limit the import of heterologous DNA. We conclude that restriction-modification systems inhibit the genomic integration of novel sequences, while they pose no barrier to homeologous recombination, which reconciles the observed stability of the H. pylori gene content and its highly recombinational population structure.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Nature Communications | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 2041-1723 | ||||
Official Date: | 22 June 2016 | ||||
Dates: |
|
||||
Volume: | 7 | ||||
Article Number: | 11995 | ||||
DOI: | 10.1038/ncomms11995 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |