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Engineering CRISPR guide RNA riboswitches for in vivo applications
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Galizi, Roberto and Jaramillo, Alfonso (2019) Engineering CRISPR guide RNA riboswitches for in vivo applications. Current Opinion in Biotechnology, 55 . pp. 103-113. doi:10.1016/j.copbio.2018.08.007
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WRAP-Engineering-CRISPR-RNA-riboswitches-Jaramillo-2018.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (861Kb) | Preview |
Official URL: https://doi.org/10.1016/j.copbio.2018.08.007
Abstract
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and multicellular organisms, the tight control of the CRISPR-editing activity remains a major challenge. Here we summarise recent developments of CRISPR and riboswitch technologies and recommend novel functionalised synthetic-gRNA (sgRNA) designs to achieve inducible and spatiotemporal regulation of CRISPR-based genetic editors in response to cellular or extracellular stimuli. We believe that future advances of these tools will have major implications for both basic and applied research, spanning from fundamental genetic studies and synthetic biology to genetic editing and gene therapy.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics | |||||||||||||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | CRISPR (Genetics), Gene editing, Riboswitches, RNA editing | |||||||||||||||||||||
| Journal or Publication Title: | Current Opinion in Biotechnology | |||||||||||||||||||||
| Publisher: | Elsevier BV | |||||||||||||||||||||
| ISSN: | 0958-1669 | |||||||||||||||||||||
| Official Date: | February 2019 | |||||||||||||||||||||
| Dates: |
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| Volume: | 55 | |||||||||||||||||||||
| Page Range: | pp. 103-113 | |||||||||||||||||||||
| DOI: | 10.1016/j.copbio.2018.08.007 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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