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The type 4 metallothionein from Brassica napus seeds folds in a metal-dependent fashion and favours zinc over other metals
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Mierek-Adamska, Agnieszka, Dąbrowskaa, Grażyna B. and Blindauer, Claudia A. (2018) The type 4 metallothionein from Brassica napus seeds folds in a metal-dependent fashion and favours zinc over other metals. Metallomics, 10 (10). pp. 1430-1443. doi:10.1039/C8MT00161H ISSN 1756-5901.
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WRAP-type-4-metallothionein-Brassica-napus-folds-zinc-metals-Blindauer-2018.pdf - Accepted Version - Requires a PDF viewer. Download (2850Kb) | Preview |
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WRAP-type-4-metallothionein-Brassica-napus-folds-zinc-metals-Blindauer-preprint-2018.pdf - Submitted Version - Requires a PDF viewer. Download (2849Kb) | Preview |
Official URL: https://doi.org/10.1039/C8MT00161H
Abstract
The problem of handling zinc in the cell is of great importance because zinc is an indis-pensable micronutrient involved in most physiological processes in all living organisms. Moreover, our understanding of mechanisms governing the discrimination between mi-cronutrients and toxic metals on the level of individual proteins to the whole-organism level is incomplete. Metallothioneins are able to bind heavy metal ions, and roles in zinc homeostasis have been proposed. Here, we have studied the in vitro and in vivo metal-binding abilities of Brassica napus type 4 metallothionein (BnMT4) and its expression in germinating seeds in response to metal treatment. Our studies on the regulation of MT4 expression by metals at early stages of ontogenic development revealed for the first time that the mRNA levels of BnMT4 were elevated in response to cadmium and zinc. Given this unexpected metalloregulation, and the dramatic differences in protein folding as de-tected by 1H NMR spectroscopy, we suggest that the BnMT4 protein may not only have a role in zinc homeostasis in early ontogenesis, but also the potential to discriminate be-tween zinc and cadmium, perhaps via differential recognition of Cd- and Zn-complexes by cellular components involved in protein turnover.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Metallothionein, Rape (Plant), Zinc -- Physiological effect, Zinc proteins, Electrospray ionization mass spectrometry, Germination, Cadmium | ||||||||
Journal or Publication Title: | Metallomics | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 1756-5901 | ||||||||
Official Date: | 17 October 2018 | ||||||||
Dates: |
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Volume: | 10 | ||||||||
Number: | 10 | ||||||||
Page Range: | pp. 1430-1443 | ||||||||
DOI: | 10.1039/C8MT00161H | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 11 October 2018 | ||||||||
Date of first compliant Open Access: | 3 September 2019 | ||||||||
RIOXX Funder/Project Grant: |
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