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Crystal structure of rhodopsin in complex with a mini-G_o sheds light on the principles of G protein selectivity
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Tsai, Ching-Ju, Pamula, Filip, Nehmé, Rony, Mühle, Jonas, Weinert, Tobias, Flock, Tilman, Nogly, Przemyslaw, Edwards, Patricia C., Carpenter, Byron, Gruhl, Thomas, Ma, Pikyee, Deupi, Xavier, Standfuss, Jörg, Tate, Christopher G. and Schertler, Gebhard F. X. (2018) Crystal structure of rhodopsin in complex with a mini-G_o sheds light on the principles of G protein selectivity. Science Advances, 4 (9). eaat7052. doi:10.1126/sciadv.aat7052 ISSN 2375-2548.
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Official URL: http://dx.doi.org/10.1126/sciadv.aat7052
Abstract
Selective coupling of G protein (heterotrimeric guanine nucleotide–binding protein)–coupled receptors (GPCRs) to specific Gα-protein subtypes is critical to transform extracellular signals, carried by natural ligands and clinical drugs, into cellular responses. At the center of this transduction event lies the formation of a signaling complex between the receptor and G protein. We report the crystal structure of light-sensitive GPCR rhodopsin bound to an engineered mini-Go protein. The conformation of the receptor is identical to all previous structures of active rhodopsin, including the complex with arrestin. Thus, rhodopsin seems to adopt predominantly one thermodynamically stable active conformation, effectively acting like a “structural switch,” allowing for maximum efficiency in the visual system. Furthermore, our analysis of the well-defined GPCR–G protein interface suggests that the precise position of the carboxyl-terminal “hook-like” element of the G protein (its four last residues) relative to the TM7/helix 8 (H8) joint of the receptor is a significant determinant in selective G protein activation.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | G proteins -- Receptors, Rhodopsin -- Structure, Cellular signal transduction, Crystallography | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Science Advances | ||||||||||||||||||||||||||||||||||||
Publisher: | American Association for the Advancement of Science | ||||||||||||||||||||||||||||||||||||
ISSN: | 2375-2548 | ||||||||||||||||||||||||||||||||||||
Official Date: | 19 September 2018 | ||||||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 4 | ||||||||||||||||||||||||||||||||||||
Number: | 9 | ||||||||||||||||||||||||||||||||||||
Article Number: | eaat7052 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1126/sciadv.aat7052 | ||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 17 October 2018 | ||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 18 October 2018 | ||||||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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