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Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro
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UNSPECIFIED (2002) Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro. HUMAN REPRODUCTION, 17 (5). pp. 1181-1188. ISSN 0268-1161.
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Abstract
BACKGROUND: This study tested factors affecting mouse follicle growth in vitro, to determine end-points marking follicle function in vitro. METHODS: Pre-antral follicles (mean 137 mum) from B6CBF1 mice were cultured in a substrate-adherent system for less than or equal to14 days. FSH (0-1000mIU/ml)day of HCG (1.5 IU/ml days 9-14) protein supplement [fetal calf serum (FCS) (x2) mouse serum (x2) hypogonadal (hpg) mouse serum or human serum albumin (HSA)] were varied. Follicle survival timing of antrum formation incidence of ovulation within 16, 24, 40 and 48 h of HCG oocyte growth were assessed. RESULTS: FSH (100 mIU/ml) produced the best antral development (P < 0.001 versus 10 1000 mIU/ml). Antra were observed from day 5. Transient antra formed occasionally in the absence of FSH. By 14 days significant senescence had occurred (P < 0.001) but the proportion of follicles ovulating within 16 h of HCG declined from day 9 onwards indicating this to be a more sensitive marker of follicle responsiveness. Optimal growth occurred in 5% FCS (x2) or hpg mouse serum although fewer follicles ovulated in hpg serum (P < 0.05). No normal growth occurred in normal mouse serum (x2) or HSA. Oocytes grew to full size within 9 days with 100 mIU/ml FSH FCS. CONCLUSIONS: These data provide sensitive end-points for assessing follicle growth in vitro.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RG Gynecology and obstetrics | ||||
Journal or Publication Title: | HUMAN REPRODUCTION | ||||
Publisher: | OXFORD UNIV PRESS | ||||
ISSN: | 0268-1161 | ||||
Official Date: | May 2002 | ||||
Dates: |
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Volume: | 17 | ||||
Number: | 5 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 1181-1188 | ||||
Publication Status: | Published |
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