Petersen, Lindsay N., Ingle, Robert A., Knight, Marc R. and Denby, Katherine J.. (2009) OXI1 protein kinase is required for plant immunity against Pseudomonas syringae in Arabidopsis. Journal of Experimental Botany, Vol.60 (No.13). pp. 3727-3735. ISSN 0022-0957

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Abstract

Expression of the Arabidopsis Oxidative Signal-Inducible1 (OXI1) serine/threonine protein kinase gene (At3g25250) is induced by oxidative stress. The kinase is required for root hair development and basal defence against the oomycete pathogen Hyaloperonospora parasitica, two separate H2O2-mediated processes. In this study, the role of OXI1 during pathogenesis was characterized further. Null oxi1 mutants are more susceptible to both virulent and avirulent strains of the biotrophic bacterial pathogen Pseudomonas syringae compared with the wild type, indicating that OXI1 positively regulates both basal resistance triggered by the recognition of pathogen-associated molecular patterns, as well as effector-triggered immunity. The level of OXI1 expression appears to be critical in mounting an appropriate defence response since OXI1 overexpressor lines also display increased susceptibility to biotrophic pathogens. The induction of OXI1 after P. syringae infection spatially and temporally correlates with the oxidative burst. Furthermore, induction is reduced in atrbohD mutants and after application of DPI (an inhibitor of NADPH oxidases) suggesting that reactive oxygen species produced through NADPH oxidases drives OXI1 expression during this plant–pathogen interaction.

Item Type:	Journal Article
Alternative Title:	Role for OXI1 in plant immunity
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Arabidopsis thaliana, Peronosporaceae, Pseudomonas syringae, Active oxygen -- Physiological effect, Transduction, Fungal diseases of plants, Plant diseases -- Genetic aspects, Plant immunology
Journal or Publication Title:	Journal of Experimental Botany
Publisher:	Oxford University Press
ISSN:	0022-0957
Date:	September 2009
Volume:	Vol.60
Number:	No.13
Page Range:	pp. 3727-3735
Identification Number:	10.1093/jxb/erp219
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	National Research Foundation (South Africa), University of Cape Town. Committee on Research (UoCT), Canon Collins Educational Trust for Southern Africa (CCETSA)
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URI:	http://wrap.warwick.ac.uk/id/eprint/1105

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