Synthesis and in vitro enzyme activity of an oxa analogue of azi-DAP
UNSPECIFIED. (2002) Synthesis and in vitro enzyme activity of an oxa analogue of azi-DAP. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 (8). pp. 1029-1035. ISSN 1472-7781Full text not available from this repository.
Official URL: http://dx.doi.org/10.1039/b201727j
The synthesis of a novel epoxide inhibitor 6 of meso-DAP epimerase was achieved. A new and convenient assay for meso-DAP epimerase was devised which is a considerable improvement on previously reported assay methods. This assay was used to determine the inhibitory properties of 6. The epoxide 6 shows limited competitive inhibition vs. meso-DAP epimerase, but clear time dependent inhibition indicative of the expected covalent attachment at the active site. Epoxide 6, although an effective inhibitor of meso-DAP epimerase, was unstable at pH 7.3 in aqueous buffer, being hydrolysed to the corresponding diol.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1|
|Publisher:||ROYAL SOC CHEMISTRY|
|Number of Pages:||7|
|Page Range:||pp. 1029-1035|
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