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Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism

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Bortolini, Christian, Kartanas, Tadas, Copic, Davor, Morales, Itzel Condado, Zhang, Yuewen, Challa, Pavan K., Peter, Quentin A. E., Jávorfi, Tamas, Hussain, Rohanah, Dong, Mingdong, Siligardi, Giuliano, Knowles, Tuomas P. J. and Charmet, Jérôme (2019) Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism. Lab on a Chip, 19 (1). pp. 50-58. doi:10.1039/C8LC00757H

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Official URL: https://doi.org/10.1039/C8LC00757H

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Abstract

Circular dichroism spectroscopy has become a powerful tool to characterise proteins and other biomolecules. For heterogeneous samples such as those present for interacting proteins, typically only average spectroscopic features can be resolved. Here we overcome this limitation by using free-flow microfluidic size separation in-line with synchrotron radiation circular dichroism to resolve the secondary structure of each component of a model protein mixture containing monomers and fibrils. To enable this objective, we have integrated far-UV compatible measurement chambers into PDMS-based microfluidic devices. Two architectures are proposed so as to accommodate for a wide range of concentrations. The approach, which can be used in combination with other bulk measurement techniques, paves the way to the study of complex mixtures such as the ones associated with protein misfolding and aggregation diseases including Alzheimer’s and Parkinson’s diseases.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH): Proteins -- Spectra, Circular dichroism
Journal or Publication Title: Lab on a Chip
Publisher: Royal Society of Chemistry
ISSN: 1473-0197
Official Date: January 2019
Dates:
DateEvent
January 2019Published
22 November 2018Available
13 November 2018Accepted
Date of first compliant deposit: 15 November 2018
Volume: 19
Number: 1
Page Range: pp. 50-58
DOI: 10.1039/C8LC00757H
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder:
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
FP7/2007-2013Seventh Framework Programmehttp://dx.doi.org/10.13039/100011102
337969H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
BB/J002119/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDWellcome Trusthttp://dx.doi.org/10.13039/100010269
UNSPECIFIEDFrances and Augustus Newman Foundationhttp://dx.doi.org/10.13039/100007898
UNSPECIFIEDLundbeckfondenhttp://dx.doi.org/10.13039/501100003554
674979-NANOTRANSH2020 Research Infrastructureshttp://dx.doi.org/10.13039/100010666
UNSPECIFIEDConsejo Nacional de Ciencia y Tecnologíahttp://dx.doi.org/10.13039/501100003141
UNSPECIFIEDGates Cambridge Trusthttp://dx.doi.org/10.13039/501100005370
EP/L015978/[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
352360246 Newton-Katip Celebi Fund British Councilhttp://dx.doi.org/10.13039/501100000308
660351-EmuCamH2020 Marie Skłodowska-Curie Actionshttp://dx.doi.org/10.13039/100010665
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