The Library
Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage
Tools
Mitchell, Daniel A., Fayter, Alice, Deller, Robert C., Hasan, Muhammad, Gutierrez-Marcos, José F. and Gibson, Matthew I. (2019) Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage. Materials Horizons, 6 (2). pp. 354-368. doi:10.1039/c8mh00727f ISSN 2051-6355.
|
PDF
WRAP-ice-recrystallization-inhibiting-polymers-proteins-freeze-Gibson-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (2498Kb) | Preview |
Official URL: https://doi.org/10.1039/C8MH00727F
Abstract
Proteins are ubiquitous in molecular biotechnology, biotechnology and as therapeutics, but there are significant challenges in their storage and distribution, with freezing often required. This is traditionally achieved by the addition of cryoprotective agents such as glycerol (or trehalose) or covalent modification of mutated proteins with cryoprotectants. Here, ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are used synergistically with poly(ethylene glycol) as an alternative to glycerol. The primary mechanism of action appears to be preventing irreversible aggregation due to ice growth. The polymer formulation is successfully used to cryopreserve a range of important proteins including insulin, Taq DNA polymerase and an IgG antibody. The polymers do not require covalent conjugation, nor modification of the protein and are already used in a wide range of biomedical applications, which will facilitate translation to a range of biologics.
Item Type: | Journal Article | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
|||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
|||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Polymers, Antifreeze proteins, Glycerin, Trehalose | |||||||||||||||
Journal or Publication Title: | Materials Horizons | |||||||||||||||
Publisher: | Royal Society of Chemistry (RSC) | |||||||||||||||
ISSN: | 2051-6355 | |||||||||||||||
Official Date: | 1 February 2019 | |||||||||||||||
Dates: |
|
|||||||||||||||
Volume: | 6 | |||||||||||||||
Number: | 2 | |||||||||||||||
Page Range: | pp. 354-368 | |||||||||||||||
DOI: | 10.1039/c8mh00727f | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 28 November 2018 | |||||||||||||||
Date of first compliant Open Access: | 28 November 2018 | |||||||||||||||
RIOXX Funder/Project Grant: |
|
|||||||||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year