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Microevolution of Neisseria lactamica during nasopharyngeal colonisation induced by controlled human infection
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Pandey, Anish, Cleary, David W., Laver, Jay R., Gorringe, Andrew, Deasy, Alice M., Dale, Adam P., Morris, Paul D., Didelot, Xavier, Maiden, Martin C. J. and Read, Robert C. (2018) Microevolution of Neisseria lactamica during nasopharyngeal colonisation induced by controlled human infection. Nature Communications, 9 (1). 4753. doi:10.1038/s41467-018-07235-5 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-018-07235-5
Abstract
Neisseria lactamica is a harmless coloniser of the infant respiratory tract, and has a mutually-excluding relationship with the pathogen Neisseria meningitidis. Here we report controlled human infection with genomically-defined N. lactamica and subsequent bacterial microevolution during 26 weeks of colonisation. We find that most mutations that occur during nasopharyngeal carriage are transient indels within repetitive tracts of putative phase-variable loci associated with host-microbe interactions (pgl and lgt) and iron acquisition (fetA promotor and hpuA). Recurrent polymorphisms occurred in genes associated with energy metabolism (nuoN, rssA) and the CRISPR-associated cas1. A gene encoding a large hypothetical protein was often mutated in 27% of the subjects. In volunteers who were naturally co-colonised with meningococci, recombination altered allelic identity in N. lactamica to resemble meningococcal alleles, including loci associated with metabolism, outer membrane proteins and immune response activators. Our results suggest that phase variable genes are often mutated during carriage-associated microevolution.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Statistics |
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Library of Congress Subject Headings (LCSH): | Neisseria, Neisseria infections -- Genetic aspects, Pathogenic microorganisms -- Genetics, Bacteria -- Evolution, Microbial mutation, Nasopharynx -- Microbiology | ||||||||||||||||||
Journal or Publication Title: | Nature Communications | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 2041-1723 | ||||||||||||||||||
Official Date: | 12 November 2018 | ||||||||||||||||||
Dates: |
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Volume: | 9 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Article Number: | 4753 | ||||||||||||||||||
DOI: | 10.1038/s41467-018-07235-5 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 29 November 2018 | ||||||||||||||||||
Date of first compliant Open Access: | 30 November 2018 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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