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Liposome-based transfection enhances RNAi and CRISPR-1 mediated mutagenesis in non-model nematode systems
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Adams, Sally, Pathak, Prachi, Shao, Hongguang, Lok, James B. and Pires-da Silva, André Francisco (2019) Liposome-based transfection enhances RNAi and CRISPR-1 mediated mutagenesis in non-model nematode systems. Scientific Reports, 9 . 483. doi:10.1038/s41598-018-37036-1 ISSN 2045-2322.
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WRAP-liposome-based-transfection-enhances-mediated-nematode-Silva-2018.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (1077Kb) |
Official URL: https://doi.org/10.1038/s41598-018-37036-1
Abstract
Nematodes belong to one of the most diverse animal phyla. However, functional genomic studies in nematodes, other than in a few species, have often been limited in their reliability and success. Here we report that by combining liposome-based technology with microinjection, we were able to establish a wide range of genomic techniques in the newly described nematode genus Auanema. The method also allowed heritable changes in dauer larvae of Auanema, despite the immaturity of the gonad at the time of the microinjection. As proof of concept for potential functional studies in other nematode species, we also induced RNAi in the free-living nematode Pristionchus pacificus and targeted the human parasite Strongyloides stercoralis.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QL Zoology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Nematodes, Liposomes, Mutagenesis, RNA interference | |||||||||||||||
Journal or Publication Title: | Scientific Reports | |||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||
ISSN: | 2045-2322 | |||||||||||||||
Official Date: | 24 January 2019 | |||||||||||||||
Dates: |
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Volume: | 9 | |||||||||||||||
Article Number: | 483 | |||||||||||||||
DOI: | 10.1038/s41598-018-37036-1 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 4 December 2018 | |||||||||||||||
Date of first compliant Open Access: | 28 January 2019 | |||||||||||||||
RIOXX Funder/Project Grant: |
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