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The binding of auxin to the Arabidopsis auxin influxtransporter, AUX1
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Carrier, David J. , Bakar, Norliza Tendot Abu , Swarup, Ranjan , Callaghan, Richard , Napier, R. (Richard), Bennett, Malcolm J. and Kerr, Ian D. (2008) The binding of auxin to the Arabidopsis auxin influxtransporter, AUX1. Plant Physiology, Vol.147 . doi:10.1104/pp.108.122044 ISSN 0032-0889.
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Official URL: http://dx.doi.org/10.1104/pp.108.122044
Abstract
The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients which control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis thaliana, has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood and we sought to address this in the present investigation. We expressed AUX1 to high levels in a baculovirus expression system and prepared membrane fragments from baculovirus-infected insect cells. These membranes proved suitable for determination of the binding of IAA to AUX1 and enabled us to determine a Kd of 2.6 µM, comparable with estimates for the Km for IAA transport. The efficacy of a number of auxin analogues and auxin transport inhibitors to displace IAA binding from AUX1 has also been determined and can be rationalized in terms of their physiological effects. Determination of the parameters describing the initial interaction between a plant transporter and its hormone ligand provides novel quantitative data for modelling auxin fluxes.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QK Botany Q Science > QH Natural history |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
| Library of Congress Subject Headings (LCSH): | Auxin | ||||
| Journal or Publication Title: | Plant Physiology | ||||
| Publisher: | American Society of Plant Biologists | ||||
| ISSN: | 0032-0889 | ||||
| Official Date: | 9 July 2008 | ||||
| Dates: |
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| Volume: | Vol.147 | ||||
| DOI: | 10.1104/pp.108.122044 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Description: | Version accepted by publisher (post-print, after peer review, before copy-editing). |
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